Reni, George; Dr.Sugunan, S(Cochin University of Science And Technology, May , 2013)
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Abstract:
Mesoporous silica nanoparticles provide a non-invasive and biocompatible
delivery platform for a broad range of applications in therapeutics, pharmaceuticals
and diagnosis. Additionally, mesoporous silica materials can be synthesized
together with other nanomaterials to create new nanocomposites, opening up a
wide variety of potential applications. The ready functionalization of silica
materials makes them ideal candidates for bioapplications and catalysis. These
properties of mesoporous silica like high surface areas, large pore volumes and
ordered pore networks allow them for higher loading of drugs or biomolecules.
Comparative studies have been made to evaluate the different procedures;
much of the research to date has involved quick exploration of new methods
and supports. Requirements for different enzymes may vary, and specific
conditions may be needed for a particular application of an immobilized
enzyme such as a highly rigid support.
In this endeavor, mesoporous silica materials having different pore size
were synthesized and easily modified with active functional groups and were
evaluated for the immobilization of enzymes. In this work, Aspergillus niger
glucoamylase, Bovine liver catalase, Candida rugosa lipase were immobilized
onto support by adsorption and covalent binding. The structural properties of
pure and immobilized supports are analyzed by various characterization
techniques and are used for different reactions of industrial applications.
Description:
Department of Applied Chemistry
Cochin University of Science and Technology
Navya, Antony; Dr.Mohanan, P V(Cochin University of Science And Technology, March 25, 2014)
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Abstract:
Several natural and synthetic supports have been assessed for their efficiency
for enzyme immobilization. Synthetic polymer materials are prepared by chemical
polymerization using various monomers. As a kind of important carrier, synthetic
polymer materials exhibit the advantages of good mechanical rigidity, high specific
surface area, inertness to microbial attack, easy to change their surface characteristics,
and their potential for bringing specific functional group according to actual needs.
Hence, they have been widely investigated and used for enzyme immobilization.
When it comes to the natural polymer materials, much attention has been paid
to cellulose and other natural polymer materials owing to their wide range of sources,
easy modification, nontoxic, and pollution-free, with a possibility of introducing wide
variety of functional groups and good biocompatible properties.
In this work report the use of synthetic polymer, polypyrrole and its
derivatives and natural polymers coconut fiber and sugarcane bagasse as supports for
Diastase α- amylase immobilization. An attempt was also made to functionalize both
synthetic and natural polymers using Amino-propyl triethoxysilane. Supports and their
immobilized forms were characterized via FT-IR, TG, SEM, XRD, BET and EDS
techniques. Immobilization parameters were also optimized so as to prepare stable
immobilized biocatalyst for starch hydrolysis.
Description:
Department of Applied Chemistry
Cochin University of Science and Technology