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Abstract:
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Several natural and synthetic supports have been assessed for their efficiency
for enzyme immobilization. Synthetic polymer materials are prepared by chemical
polymerization using various monomers. As a kind of important carrier, synthetic
polymer materials exhibit the advantages of good mechanical rigidity, high specific
surface area, inertness to microbial attack, easy to change their surface characteristics,
and their potential for bringing specific functional group according to actual needs.
Hence, they have been widely investigated and used for enzyme immobilization.
When it comes to the natural polymer materials, much attention has been paid
to cellulose and other natural polymer materials owing to their wide range of sources,
easy modification, nontoxic, and pollution-free, with a possibility of introducing wide
variety of functional groups and good biocompatible properties.
In this work report the use of synthetic polymer, polypyrrole and its
derivatives and natural polymers coconut fiber and sugarcane bagasse as supports for
Diastase α- amylase immobilization. An attempt was also made to functionalize both
synthetic and natural polymers using Amino-propyl triethoxysilane. Supports and their
immobilized forms were characterized via FT-IR, TG, SEM, XRD, BET and EDS
techniques. Immobilization parameters were also optimized so as to prepare stable
immobilized biocatalyst for starch hydrolysis. |