Sareen Sarah, John; Reed, Robert H.; Bright Singh, I S(Cochin University Of Science And Technology, December , 2014)
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Abstract:
A study was undertaken to isolate phytase producers from
environment and to segregate the most highly efficient phytase producer and to
develop a bioprocess technology for commercial application. During this
process, a potential phytase producer Bacillus MCCB 242 was isolated and
characterized phenotypically and genotypically. Subsequently, phytase production
was optimized, the enzyme purified and characterized and an appropriate
downstream process also could be standardized.Precisely, through this work an environmental isolate Bacillus MCCB 242 could be brought out as phytase producer for commercial application. The enzyme production could be optimized and characterized, and an appropriate
downstream process standardized. Cytotoxicity studies revealed the enzyme
safe for feed application, especially in fish.