Cini, Achuthan; Bright Singh, I S(Schoolof Environmental Studies, 2000)
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Abstract:
In the present study the development of bioreactors for nitrifying water in closed system hatcheries of penaeid and non-penaeid prawns. This work is an attempt in this direction to cater to the needs of aquaculture industry for treatment and remediation of ammonia and nitrate in penaeid and non-penaeid hatcheries, by developing nitrifying bacteria allochthonous to the particular environment under consideration, and immobilizing them on an appropriately designed support materials configured as reactors. Ammonia toxicity is the major limiting factors in penaeid and non-penaeid hatchery systems causing lethal and sublethal effects on larvae depending on the pH values. Pressing need of the aquaculture industry to have a user friendly and economically viable technology for the removal of ammonia, which can be easily integrated to the existing hatchery designs without any major changes or modifications. Only option available now is to have biological filters through which water can be circulated for the oxidation of ammonia to nitrate through nitrite by a group of chemolithotrophs known as nitrifying bacteria. Two types of bioreactors have been designed and developed. The first category named as in situ stringed bed suspended bioreactor(SBSBR) was designed for use in the larval rearing tanks to remove ammonia and nitrite during larval rearing on a continuous basis, and the other to be used for nitrifying freshly collected seawater and spent water named as ex situ packed bed bioreactior(PBBR). On employing the two reactors together , both penaeid and non-penaeid larval rearing systems can be made a closed recirculating system at least for a season. A survey of literature revealed that the in situ stringed bed suspended reactor developed here is unique in its design, fabrication and mode of application.
Sunil Kumar, G; Bright Singh, I S(School of Environment Studies, Faculty of Environment Studies, 2000)
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Abstract:
The present work deals with the development of primary cell culture and diploid cell lines from two fishes, such as Poecilia reticulata and Clarias gariepinus. The greatest difficulty experienced was the avoidance of bacterial and fungi contamination. Three types of cell cultures are commonly developed, primary cell culture, diploid cell lines and heteroploid cell lines. Primary cell culture obtained from the animal tissues that have been cultivated in vitro for the first time. They are characterized by the same chromosome number as parent tissue, cultivated in vitro for the first time, have wide range of virus susceptibility, usually not malignant, six chromatin retarded and do not grow as suspension cultures. Diploid cell lines arise from a primary cell culture at the time of subculturing. Diploid cell lines commercially used in virology are W1-38 (human embryonic lung), W1-26 (human embryonic lung) and HEX (Human embryonic kidney). Heteroploid cell lines have been subcultivated with less than 75% of the cells in the population having a diploid chromosome constitution. Tissue cultures have been extensively used in biomedical research. The main applications are in three areas, Karyological studies, Identification and study of hereditary metabolic disorders and Somatic cell genetics. Other applications are in virology and host-parasite relationships. In this study an attempt was made to preserve the ovarian tissue at low temperature in the presence of cryoprotectants so that the tissue can be retrieved at any time and a cell culture could be developed.
Jayesh, P; Dr.Bright Singh,I S(Cochin University of Science and Technology, September , 2012)
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Abstract:
Unveiling the molecular and regulatory mechanisms that prevent in vitro
transformation in shrimp remains elusive in the development of continuous cell
lines, with an arduous history of over 25 years (Jayesh et al., 2012). Despite
presenting challenges to researchers in developing a cell line, the billion dollar
aquaculture industry is under viral threat. In addition, the regulatory mechanisms
that prevent in vitro transformation and carcinoma in shrimps might provide new
leads for the development of anti-ageing and anti-cancer interventions in human
(Vogt, 2011) and in higher vertebrates. This highlights the importance of
developing shrimp cell lines, to bring out effective prophylactics against shrimp
viruses and for understanding the mechanism that induce cancer and ageing in
human.. Advances in molecular biology and various gene transfer technologies for
immortalization of cells have resulted in the development of hundreds of cell lines
from insects and mammals, but yet not a single cell line has been developed from
shrimp and other marine invertebrates. With this backdrop, the research described
in this thesis attempted to develop molecular tools for induced in vitro
transformation in lymphoid cells from Penaeus monodon and for the development
of continuous cell lines using conventional and novel technologies to address the
problems at cellular and molecular level.
Description:
National Centre for Aquatic Animal
Health, School of Environmental Studies, Cochin University of Science and
Technology
Manju, N J; Dr.Bright Singh,I S(Cochin University of Science and Technology, October , 2007)
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Abstract:
This thesis entitled Development of nitrifying ans photosynthetic sulfur bacteria based bioaugmentation systems for the bioremediation of ammonia and hydregen sulphide in shrimp culture.
the thesis is to propose a sustainable, low cost option for the mitigation of toxic ammonia and hydrogen sulphide in shrimp culture systems. Use of ‘bioaugmentors’ as pond additives is an emerging field in aquaculture. Understanding the role of organisms involved in the ‘bioaugmentor’ will obviously help to optimize conditions for their activity.The thesis describes the use of wood powder immobilization of nitrifying consortia.Shrimp grow out systems are specialized and highly dynamic aquaculture production units which when operated under zero exchange mode require bioremediation of ammonia, nitrite nitrogen and hydrogen sulphide to protect the crop. The research conducted here is to develop an economically viable and user friendly technology for addressing the above problem. The nitrifying bacterial consortia (NBC) generated earlier (Achuthan et al., 2006) were used for developing the technology.Clear demonstration of better quality of immobilized nitrifiers generated in this study for field application.
Description:
National Centre for Aquatic Animal Health, School of Environmental
Studies, Cochin University of Science and Technology
Haseeb, M; Dr.Bright Singh,I S(Cochin University Of Science And Technology, November , 2012)
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Abstract:
Aquaculture is one of the fastest growing food sectors in the world.
Amongst the various branches of aquaculture, shrimp culture has expanded
rapidly across the globe because of its faster growth rate, short culture period,
high export value and demand in the International market. Indian shrimp
farming has experienced phenomenal development over the decades due to its
excellent commercial viability. Farmers have adopted a number of innovative
technologies to improve the production and to maximize the returns per unit
area. The culture methods adopted can be classified in to extensive, modified
extensive and semi intensive based on the management strategies adopted in
terms of pond size, stocking density, feeding and environmental control. In all
these systems water exchanges through the natural tidal effects, or pump fed
either from creek or from estuaries is a common practice. In all the cases, the
systems are prone to epizootics due to the pathogen introduction through the
incoming water, either brought by vectors, reservoir hosts, infected tissue
debris and free pathogens themselves. In this scenario, measures to prevent the
introduction of pathogen have become a necessity to protect the crop from the
onslaught of diseases as well as to prevent the discharge of waste water in to
the culture environment.The present thesis deals with Standardization of bioremediation technology for zero water exchange shrimp culture system
Description:
School of Environmental Studies,Cochin University of Science and Technology
Luckose, C K; Dr.Chandrasekharan Pillai, N(Cochin University of Science and Technology, September , 1992)
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Abstract:
When we consider Kerala and Karnataka States according
to their levels of decentralisation. Kerala is at the beginning
of the scale of decentralisation whereas Kamataka has moved far
ahead along this scale. Therefore I in order to conduct a comparative
study of the SUbject under analysis t Kamataka has been selected
owing to the fact that it is in an advanced stage in the experience
of district planning compared to Kerala , Karnataka could successfully
implement district planning and it is me of the pioneering states
in this regard. But Kerala has not gained much experience in
the field of decentralised district planning till now. Furthermore
Kerala and Kamataka states are selected for the present study
due to operational reasons I besides the author I s familiarity with
the socia-economic conditions of these states. Thus. an analysis
of the district planning experience of Kamataka will provide
constructive and valuable information. which will be of great
importance to Kerala State, which is now aspiring to introduce
ful.I-f'Iedge district planning by constituting elected District Coancils
in every district of Kerala. Moreover. the findings and policy
implications of the present study will be of immense help to
planners, politicians. administrators, academicians and people
at large.
Description:
School of Management Studies, Cochin University of Science & Technology
Magesh, G; Dr. Menon, A R R(Cochin University of Science And Technology, February 12, 2014)
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Abstract:
mbikulam Tiger Reserve of Western Ghats using Geospatial technology. The major objectives of the study are Land use land cover mapping (LULC) and Phytodiversity analysis. Satellite data was used to map the land use / land cover using supervised classification techniques in Erdas imagine. The change for a period of 32 years was assessed using the multi-temporal satellite datasets from Landsat MSS (1973), Landsat TM (1990), and IRS P6 LISS III (2005). A geospatial approach was used for the land cover analysis. Digital elevation models, Satellite imageries and SOI topo sheets were the data sets used in the analysis. Vegetation sampling plots distributed over the different forest types were enumerated and studied for Phytodiversity analysis.
Description:
Dept. of GIS & Remote Sensing.
Forest Management and Information System Division,Kerala Forest Research Institute
Kanakavalli,Susarla S; Dr.Balakrishnan, K P(Cochin University of Science and Technology, March 10, 1987)
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Abstract:
The extraction and use of metals has been the mainstay for the sustained development and progress of a nation. Metals, though fairly stable in the natural environment are found in trace quantities in water bodies. Attention has therefore been focused to identify the metals that impair the water quality. In the last few decades the concern about the fate of these metals in the aquatic system has been gaining momentum, particularly in the industrial belts. The disasters caused by metal poisoning in recent times have prompted an indepth study of the interaction of metals with aquatic biota. Kerala, basically an agriculture oriented state has witnessed the upsurgence of various industries as a part of the nationwide economic development programme. Cochin has been identified as the industrial capital of the state.The present study is an attempt towards a better understanding of the metal-phytoplankton interactions with special reference to the physiological changes in the species. various parameters such as temperature, salinity, pH, nutrients, number of cells, photosynthetic pigments, carbohydrates, protein and lipid are studied to highlight the complexity of metal..phytoplankton interaction
Description:
School of Environmental Studies,
Cochin University of Science and Technology
Sreebha, S; Dr. Padmalal, D(Cochin University of Science and Technology, November , 2008)
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Abstract:
This thesis Entitled Environmental impact of Sand Mining :A case Study in the river catchments of vembanad lake southwest india.The entire study is addressed in nine chapters. Chapter l deals with the general introduction about rivers, problems of river sand mining, objectives, location of the study area and scope of the study. A detailed review on river classification, classic concepts in riverine studies, geological work of rivers and channel processes, importance of river ecosystems and its need for management are dealt in Chapter 2. Chapter 3 gives a comprehensive account of the study area - its location, administrative divisions, physiography, soil, geology, land use and living and non-living resources. The various methods adopted in the study are dealt in Chapter 4. Chapter 5 contains river
characteristics like drainage, environmental and geologic setting, channel characteristics, river discharge and water quality of the study area. Chapter 6 gives an account of river sand mining (instream and floodplain mining) from the study area. The various environmental problems of river sand mining on the land adjoining the river banks, river channel, water, biotic and social / human environments of the area and data interpretation are presented in Chapter 7. Chapter 8 deals with the Environmental Impact Assessment (EIA) and Environmental Management Plan (EMP) of sand mining from the river catchments of Vembanad lake.
Description:
Environmental Sciences Division, Centre for
Earth Science Studies
Latha, C; Dr.Ammini, Joseph(Cochin University of Science and Technology, April , 1998)
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Abstract:
In the last decades considerable headway has been made in research and development
of phyto-chemical pesticides. The most notable recent success is the commercial
development of neem products for insect control. The present investigation on
Environmentally Compatible Phyto-Chemical Larvicides for Mosquito Control was
undertaken to identify plants of the locality with potential larvicidal activity on mosquito
larvae. This has been achieved by screening 17 plant species against four mosquito species.
The observation and data are compiled in six chapters .
Description:
School of Environmental Studies, Cochin University of Science and Technology
Benjamin, P V; Dr.Balakrishnan, K P; Dr.Harindranathan Nair, M V(Cochin University Of Science And Technology, March , 1998)
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Abstract:
In the case of urban centres of the developing countries, corrective measures
for the environmental consequences of spontaneous or wrongly planned
developments are often prohibitively costly. Hence environmentally planned
development alone appears to be the solution for which, a compre-hensive
assessment of all the resources is an essential pre—requisite. An under-standing
of the prevailing environmental conditions is essential for the effective
management and execution of programmes for sustainable development.
The present work is a modest attempt at assessing the environmental
resources of Cochin, the industrial and business capital of Kerala and a fast
developing metropolis.
Description:
School of Environmental Studies, Cochin University of Science
and Technology
Jitha, G; Dr.Ammini, Joseph(Cochin University of Science and Technology, October , 2005)
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Abstract:
The microalgae gained importance as food and feed as well as source of fine
chemicals since the l960’s. Spirulina became the trend setter due to its easily
culturable properties as well as nutritional composition. A rapid expansion of
microalgal industry occurred in the Asia-Pacific region as microalgae came to
stay as a health food supplement.
Microalgae have been an integral component of oxidation ponds usually
incorporated with wastewater treatment. Over the last few decades, efforts
have been made to apply intensive microalgal cultures to perform biological
tertiary treatment of secondary effluents. Given the limited number of species
still available for commercial exploitation, it is imperative to isolate and
cultivate those photosynthetic organisms with high growth rate and biomass
accumulation, which could play the dual role of cleaning the wastewater and
also providing useful biomass. This has been the objective of this study ie.
0 To develop pure cultures of local isolates of Cyanobacteria for
extraction of biochemicals of commercial value
0 To couple biomass production with effluent treatment
Description:
School of Environmental Studies, Cochin University of Science And Technology