Chandrasekaran, M; Nagendra, Prabhu G(Elsevier, 1997)
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Abstract:
Process parameters influencing e-glutaminase production by marine Vibrio costicola in solid state fermentation
(SSF) using polystyrene as an inert support were optimised. Maximal enzyme yield (157 U/g dry
substrate) was obtained at 2% (w/w) t:glutamine, 35°C and pH 7.0 after 24 h. Maltose and potassium
dihydrogen phosphate at 1% (w/w) concentration enhanced enzyme yield by 23 and 18%, respectively, while
nitrogen sources had an inhibitory effect. Leachate with high specific activity for glutaminase (4.2 U/mg
protein) and low viscosity (0-966 Ns/m 2) was recovered from the polystyrene SSF system
Description:
Process Biochemistry, Vol. 32, No. 4, pp. 285-289, 1997
Maya Paul, T; Dr.Saramma, A V(Cochin University of Science & Technology, 2005)
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Abstract:
This thesis presents a detailed account of a cost - effective approach
towards enhanced production of alkaline protease at profitable levels using
different fermentation designs employing cheap agro-industrial residues. It
involves the optimisation of process parameters for the production of a
thermostable alkaline protease by Vibrio sp. V26 under solid state,
submerged and biphasic fermentations, production of the enzyme using cell
immobilisation technology and the application of the crude enzyme on the
deproteinisation of crustacean waste.The present investigation suggests an economic move towards
Improved production of alkaline protease at gainful altitudes employing
different fermentation designs utilising inexpensive agro-industrial residues.
Moreover, the use of agro-industrial and other solid waste substrates for
fermentation helps to provide a substitute in conserving the already dwindling
global energy resources. Another alternative for accomplishing economically
feasible production is by the use of immobilisation technique. This method
avoids the wasteful expense of continually growing microorganisms. The
high protease producing potential of the organism under study ascertains
their exploitation in the utilisation and management of wastes. However,
strain improvement studies for the production of high yielding variants using
mutagens or by gene transfer are required before recommending them to
Industries.Industries, all over the world, have made several attempts to exploit
the microbial diversity of this planet. For sustainable development, it is
essential to discover, develop and defend this natural prosperity. The
Industrial development of any country is critically dependent on the intellectual
and financial investment in this area. The need of the hour is to harness the
beneficial uses of microbes for maximum utilisation of natural resources and
technological yields. Owing to the multitude of applications in a variety of
industrial sectors, there has always been an increasing demand for novel
producers and resources of alkaline proteases as well as for innovative
methods of production at a commercial altitude. This investigation forms a
humble endeavour towards this perspective and bequeaths hope and
inspiration for inventions to follow.
Chandrasekaran, M; Nagendra, Prabhu G(Tecpar, 1999)
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Abstract:
L - Glutaminase, a therapeutically and industrially important enzyme, was produced from marine Vibrio costicola
by a novel solid state fermentation process using polystyrene beads as inert support. The new fermentation system
offered several advantages over the conventional systems, such as the yield of leachate with minimum viscosity and
high specific activity for the target product besides facilitating the easy estimation of biomass. The enzyme thus
produced was purified and characterised. It was active at physiological pH, showed high substrate specificity
towards L - glutamine and had a Km value of 7.4 x 10-2 M. It also exhibited high salt and temperature tolerance
indicating good scope for its industrial and therapeutic applications
Description:
Brazilian Archives of Biology and Technology 42(3),pp-363-368
Chandrasekaran, M; Suresh, P V(Rapid Science Publishers, 1998)
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Abstract:
Prawn waste, a chitinous solid waste of the shell®sh processing industry, was used as a substrate for chitinase
production by the marine fungus Beauveria bassiana BTMF S10, in a solid state fermentation (SSF) culture.
The process parameters in¯uencing SSF were optimized. A maximum chitinase yield of 248.0 units/g initial dry
substrate (U/gIDS) was obtained in a medium containing a 5:1 ratio (w/v) of prawn waste/sea water, 1% (w/w)
NaCl, 2.5% (w/w) KH2PO4, 425±600 lm substrate particle size at 27 °C, initial pH 9.5, and after 5 days of incubation.
The presence of yeast extract reduced chitinase yield. The results indicate scope for the utilization of shell®sh
processing (prawn) waste for the industrial production of chitinase by using solid state fermentation.
Description:
World Journal of Microbiology & Biotechnology, Vol 14, 1998