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Abstract:
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This thesis presents a detailed account of a cost - effective approach
towards enhanced production of alkaline protease at profitable levels using
different fermentation designs employing cheap agro-industrial residues. It
involves the optimisation of process parameters for the production of a
thermostable alkaline protease by Vibrio sp. V26 under solid state,
submerged and biphasic fermentations, production of the enzyme using cell
immobilisation technology and the application of the crude enzyme on the
deproteinisation of crustacean waste.The present investigation suggests an economic move towards
Improved production of alkaline protease at gainful altitudes employing
different fermentation designs utilising inexpensive agro-industrial residues.
Moreover, the use of agro-industrial and other solid waste substrates for
fermentation helps to provide a substitute in conserving the already dwindling
global energy resources. Another alternative for accomplishing economically
feasible production is by the use of immobilisation technique. This method
avoids the wasteful expense of continually growing microorganisms. The
high protease producing potential of the organism under study ascertains
their exploitation in the utilisation and management of wastes. However,
strain improvement studies for the production of high yielding variants using
mutagens or by gene transfer are required before recommending them to
Industries.Industries, all over the world, have made several attempts to exploit
the microbial diversity of this planet. For sustainable development, it is
essential to discover, develop and defend this natural prosperity. The
Industrial development of any country is critically dependent on the intellectual
and financial investment in this area. The need of the hour is to harness the
beneficial uses of microbes for maximum utilisation of natural resources and
technological yields. Owing to the multitude of applications in a variety of
industrial sectors, there has always been an increasing demand for novel
producers and resources of alkaline proteases as well as for innovative
methods of production at a commercial altitude. This investigation forms a
humble endeavour towards this perspective and bequeaths hope and
inspiration for inventions to follow. |