Process Optimization for Mass Production of Marine Yeast Candida sp. S 27 and its Nutritional Characterization

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Process Optimization for Mass Production of Marine Yeast Candida sp. S 27 and its Nutritional Characterization

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dc.contributor.author Simi Joseph, P
dc.contributor.author Dr.Rosamma, Philip
dc.date.accessioned 2011-10-15T10:17:25Z
dc.date.available 2011-10-15T10:17:25Z
dc.date.issued 2009-07
dc.identifier.uri http://dyuthi.cusat.ac.in/purl/2363
dc.description Dept. of Marine Biology, Microbiology and Biochemistry, Cochin University of Science and Technology, en_US
dc.description.abstract The main source of protein for human and animal consumption is from the agricultural sector, where the production is vulnerable to diseases, fluctuations in climatic conditions and deteriorating hydrological conditions due to water pollution. Therefore Single Cell Protein (SCP) production has evolved as an excellent alternative. Among all sources of microbial protein, yeast has attained global acceptability and has been preferred for SCP production. The screening and evaluation of nutritional and other culture variables of microorganisms are very important in the development of a bioprocess for SCP production. The application of statistical experimental design in bioprocess development can result in improved product yields, reduced process variability, closer confirmation of the output response to target requirements and reduced development time and overall cost.The present work was undertaken to develop a bioprocess technology for the mass production of a marine yeast, Candida sp.S27. Yeasts isolated from the offshore waters of the South west coast of India and maintained in the Microbiology Laboratory were subjected to various tests for the selection of a potent strain for biomass production. The selected marine yeast was identified based on ITS sequencing. Biochemical/nutritional characterization of Candida sp.S27 was carried out. Using Response Surface Methodology (RSM) the process parameters (pH, temperature and salinity) were optimized. For mass production of yeast biomass, a chemically defined medium (Barnett and Ingram, 1955) and a crude medium (Molasses-Yeast extract) were optimized using RSM. Scale up of biomass production was done in a Bench top Fermenter using these two optimized media. Comparative efficacy of the defined and crude media were estimated besides nutritional evaluation of the biomass developed using these two optimized media. en_US
dc.language.iso en en_US
dc.publisher Cochin University of Science & Technology en_US
dc.subject marine yeast Candida sp. S 27 en_US
dc.subject Nutritional Characterization en_US
dc.subject molecular characterization en_US
dc.subject salinity en_US
dc.subject biomass production en_US
dc.subject Optimization process en_US
dc.title Process Optimization for Mass Production of Marine Yeast Candida sp. S 27 and its Nutritional Characterization en_US
dc.type Thesis en_US
dc.contributor.faculty Marine Sciences en_US


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