Ashly, P C; Dr.Mohanan, P V(Cochin University of Science and Technology, April , 2009)
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Abstract:
This work was focused to study the immobilization of enzymes on polymers. A
large range of polymer matrices have been employed as supports for enzyme
immobilization. Here polyaniline (PAN!) and poly(0~toluidine) (POT) were used as
supports. PANI and POT provides an excellent support for enzyme immobilization by
virtue of its facile synthesis, superior chemical and physical stabilities, and large retention
capacity. We selected industrially important starch hydrolyzing enzymes a-amylase and
glucoamylase for the study. In this work the selected enzymes were immobilized via
adsorption and covalent bonding methods.To optimize the catalytic efficiency and stability of the resulting biocatalysts, the
attempt was made to understand the immobilization effects on enzymatic properties. The
effect of pH of the immobilization medium, time of immobilization on the immobilization
efficiency was observed. The starch hydrolyzing activity of free 0:-amylase and
glucoamylase were compared with immobilized forms. Immobilization on solid supports changes the microenvironment of the enzyme there by influences the pH and temperature
relationship on the enzymatic activity. Hence these parameters also optimized. The
reusability and storage stability of immobilized enzymes an important aspect from an
application standpoint, especially in industrial applications. Taking in to consideration of
this, the reusability and the long tenn storage stability of the immobilized enzyme
investigated.
Description:
Department of Applied Chemistry, Cochin
University of Science and Technology