Abstract: | The nearshore marine ecosystem is a dynamic environment impacted by many activities, especially the coastal waters and sediments contiguous to major urban areas. Although heavy metals are natural constituents of the marine environment, inputs are considered to be conservative pollutants and are potentially toxic, accumulate in the sediment, are bioconcentrated by organisms and may cause health problems to humans via the food chain. A variety of metals in trace amounts are essential for biological processes in all organisms, but excessive levels can be detrimental by acting as enzyme inhibitors. Discharge of industrial wastewater, agriculture runoff and untreated sewage pose a particularly serious threat to the coastal environment of Kerala, but there is a dearth of studies in documenting the contaminant metals. This study aimed principally to assess such contamination by examining the results of heavy metal (Cu, Pb, Cr, Ni, Zn, Cd and Hg) analysis in seawater, sediment and benthic biota from a survey of five transects along the central and northern coast of Kerala in 2008 covering a 10.0 km stretch of near shore environment in each transect. Trophic transfer of metal contaminants from aquatic invertebrates to its predators was also assessed, by employing a suitable benthic food chain model in order to understand which all metals are undergoing biotransference (transfer of metals from a food source to consumer).The study of present contamination levels will be useful for potential environmental remediation and ecosystem restoration at contaminated sites and provides a scientific basis for standards and protective measures for the coastal waters and sediments. The usefulness of biomonitor proposed in this study would allow identification of different bioavailable metals as well as provide an assessment of the magnitude of metal contamination in the coastal marine milieu. The increments in concentration of certain metals between the predator and prey discerned through benthic food chain can be interpreted as evidence of biotransference. |
Description: | School of Environmental Studies Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/3746 |
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Dyuthi-T1707.pdf | (8.182Mb) |
Abstract: | Everywhere, on the coastal belt it is proved without doubt that the pristine ground water quality was severely deteriorated after the 26 December 2004 Indian Ocean Tsunami. But how far is more relevant, as it is decided by the so-called pre-tsunamic situation of the region. In water quality studies it is this reference finger print which earmarks regional ground water chemistry based on which the monthly variability could rationally be interpreted. This Ph D thesis comprises the testing and evaluation of the facts: whether there is any significant difference in the water quality parameters under study between stations and between months in Tsunami Affected Dug Wells (TADW). Whether the selected water quality parameters vary significantly from BIS and WHO standards. Whether the water quality index (WQI) differ significantly between Tsunami Affected Dug Wells (TADW) and Bore Wells (BW). Whether there is any significant difference in the water quality parameters during December 2005 and December 2008. Is there any significant change in the Water Quality Parameters before 2001 and after tsunami (2005) in TADW. |
Description: | School of Environmental Studies, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/3708 |
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Dyuthi-T1666.pdf | (13.18Mb) |
Abstract: | This thesis Entitled Biology of Decapod crustaceans in different Environmental conditions. Prawn culture is practiced in Kerala in two types of fields namely seasonal and perennial; In seasonal fields paddy is grown during the monsoon period and prawns are cultured during the rest of the year. when compared to seasonal fields; perennial fields are subjected to much fluctuations in its environmental characteristics. The perennial fields were found more productive than seasonal fields. The benthic production in perronial fields were almost double that of seasonal fields. But in the matter of species diversity both fields were equal. Seasonal changes were observéd in the species abundance of benthic organisms in both the seasonal and perennial fields. Both isometric and allometric growth were noticed in P. indicus .But during most of the months the growth was near to isometric. The condition of the prawn also fluctuated between better and poor.During most of the months the prawn in perennial fields wore in good condition while in the seasonal fields the condition changed from year to year. This indicates that the perennial field provides a better habitat for P. indicus. |
Description: | School of Environmental Studies, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/3048 |
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Dyuthi-T1022.pdf | (10.20Mb) |
Abstract: | A study was undertaken to isolate phytase producers from environment and to segregate the most highly efficient phytase producer and to develop a bioprocess technology for commercial application. During this process, a potential phytase producer Bacillus MCCB 242 was isolated and characterized phenotypically and genotypically. Subsequently, phytase production was optimized, the enzyme purified and characterized and an appropriate downstream process also could be standardized.Precisely, through this work an environmental isolate Bacillus MCCB 242 could be brought out as phytase producer for commercial application. The enzyme production could be optimized and characterized, and an appropriate downstream process standardized. Cytotoxicity studies revealed the enzyme safe for feed application, especially in fish. |
URI: | http://dyuthi.cusat.ac.in/purl/4994 |
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Dyuthi-T2063.pdf | (24.75Mb) |
Description: | National Centre for Aquatic Animal Health, School of Environmental Studies, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/2971 |
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Dyuthi-T0960.pdf | (7.305Mb) |
Abstract: | The objective of this research is to study the feasibility of bioremediating the oily sludge from a refinery site. Three different methods of waste treatment were tried i.e. phytoremediation, land farming and microbial enhanced oil separation in laboratory scale treatment systems. A multiprocess approach by combination of phytoremediation, biostimulation and microbial enhanced oil separation is also presented. The methods of analysis, experimental procedure, and results are incorporated into five chapters of this thesis entitled "Bioremediation of petroleum sludge through phytoremediation, land farming and microbial enhanced oil separation. |
Description: | School of Environmental Studies, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/2967 |
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Dyuthi-T0956.pdf | (10.18Mb) |
Abstract: | Carbon storage potential of teak plantation was estimated by studying plantations in Nilambur undergoing prescribed thinning schedules. Nilambur in Kerala State has the reputation of establishing the first teak plantation in India. The area has a humid tropical climate with around 300 cm annual rainfall received from the two monsoons. The soil is well drained coarse textured oxisol with high content of sesquioxides. An average teak tree at Nilambur was found to attain a height of 6.93 m and dbh of 6.3 cm at 5 year which was seen to increase to 22.83 m and 45.85 cm, respectively at the final felling stage of 50 years. Biomass was found to increase from 65.38 kg tree-1 at the first stage to 1085.70 kg tree-1 at the final stage of felling. Significant increase in growth and biomass production was noted after 30th year of plantation.Carbon sequestration in various compartments of teak followed the pattern bole > branch > root > bark in the initial stages and bole > root > branch > bark in the latter stages. Carbon sequestration increased with age and at 50 years 332.88 kg tree- 1 carbon was found to be stored in bole, 60.63 in branch, 80.06 in root and 26.57 kg tree-1 in bark compartment giving a total of 508.14 kg tree-1 of carbon.Allometric models to predict carbon sequestration with height and dbh as independent variable and carbon sequestered as dependent variable were tested to obtain the best fit model. The best regression model for predicting carbon sequestered in the bole compartment was √Y = 1.502 + 0.344 D, that for bark √Y = 1.163 + 0.082 D, for branch ln Y =1.308 lnD-1.116, for root √Y = 0.858 + 0.170 D, for above ground compartment √Y = 2.113 + 0.379 D and that for predicting the total carbon sequestered in the teak in all its vegetative parts was √Y = 2.289 + 0.415 D. Carbon sequestration potential of teak plantations in Kerala was calculated based on the estimated carbon sequestration at prescribed felling stages and the area prescribed for felling in 2014. The calculated figure was 0.21 million tons of carbon which was equivalent to Certified Emission Reduction (CER) potential of 0.81 million units corresponding to 61.48 crores of rupees at current exchange rates |
URI: | http://dyuthi.cusat.ac.in/purl/5165 |
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Dyuthi-T2199.pdf | (10.93Mb) |
Description: | National Centre for Aquatic Animal Health, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/2485 |
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Dyuthi-T0668.pdf | (12.76Mb) |
Abstract: | The research work which was carried out to characterization of wastes from natural rubber and rubber wood processing industries and their utilization for biomethanation. Environmental contamination is an inevitable consequence of human activity. The liquid and solid wastes from natural rubber based industries were: characterized and their use for the production of biogas investigated with a view to conserve conventional energy, and to mitigate environmental degradation.Rubber tree (flevea brasiliensis Muell. Arg.), is the most important commercial source of natural rubber and in india. Recently, pollution from the rubber processing factories has become very serious due to the introduction of modern methods and centralized group processing practices.The possibility of the use of spent slurry as organic manure is discussed.l0 percent level of PSD, the activity of cellulolytic, acid producing,proteolytic, lipolytic and methanogenic bacteria were more in the middle stage of methanogenesis.the liquid wastes from rubber processing used as diluents in combination with PSD, SPE promoted more biogas production with high methane content in the gas.The factors that favour methane production like TS, VS, cellulose and hemicellulose degradation were favoured in this treatment which led to higher methane biogenesis.The results further highlight ways and means to use agricultural wastes as alternative sources of energy. |
Description: | Environmental Studies, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/3059 |
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Dyuthi-T1033.pdf | (4.264Mb) |
Abstract: | The tiniest Union territory of India, Lakshadweep, is an archipelago, with an area of 32 Sq. km. consisting of 12 atolls, three reefs and five submerged banks, lies between 8° and 12°30'N latitudes and 71° and 74" E longitudes. It is one of the most important and critical territories of India from economic and defence point of view. Specialised environment having typical geological set up, Lakshadweep is ecologically sensitive to even slight climatic or anthropogenic interference. Pollution of coastal seas, over exploitation and contamination of the fresh water sources are thus become great concerns to the existence of the island. Typical geological set up and interference cause threat to the ecology of the fragile environment and resources of the island as well as its resources. Marine pollution and ground water contamination are concerns in this regard. Even though attentions were made to assess the physico—chemical and bacteriological status of the marine and groundwater systems separately, an integrated approach has not been evolved. The present study with its broad objectives is attempted for an integrated assessment of microbiological, physicochemical and biological characteristics of the surrounding seawater and microbiological and physico—chemical characteristics of the ground water in Kavaratti island. The entire study has been organised in 4 chapters |
Description: | Chemical Sciences Division, Centre for Earth Science Studies |
URI: | http://dyuthi.cusat.ac.in/purl/3368 |
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Dyuthi-T1345.pdf | (6.223Mb) |
Abstract: | This thesis Entitled Colour removal from dye house effluents using zero valent iron and fenton oxidation.Findings reported on kinetic profile during oxidation of dyes with Fenton’s reagent are in good agreement with observations of earlier workers on other organic substrates. This work goes a step further. Critical concentration of the dye at which the reaction mechanism undergoes transition has been identified.The oxidation of Reactive Yellow showed that the initial rates for decolorization increased linearly with an increase in hydrogen peroxide concentration over the range studied. Fenton oxidation of all dyes except Methylene Blue showed that the initial rates increased linearly with an in the ferrous sulphate concentration. This increase was observed only up to an optimum concentration beyond which further increase resulted in a decrease in the initial rates. Variation of initial rates with Ferrous sulphate concentration resulted in a linear plot passing through the origin indicating that the reaction is first order with respect to ferrous sulphate. |
Description: | School of Environmental Studies, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/3149 |
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Dyuthi-T1123.pdf | (11.54Mb) |
Abstract: | Spike disease in sandal is generally diagnosed by the manifestation of external symptoms. Attempts have been made to detect the diseased plants by determining the length/breadth ratio of leaves (lyengar, 1961) and histochemical tests using Mann's stain (Parthasarathi et al., 1966), Dienes' stain (Ananthapadmanabha et a/., 1973) aniline blue and Hoechst 33258 (Ghosh et a/., 1985, Rangaswamy, 1995). But most of these techniques are insensitive, indirect detection methods leading to misinterpretation of results. Moreover, to identify disease resistant sandal trees, highly sensitive techniques are needed to detect the presence of the pathogen. In sandal forests, several host plants of sandal like Zizyphus oenop/ea (Fig. 1.3) also exhibit the yellows type disease symptoms. Immunological and molecular assays have to be developed to confirm the presence of sandal spike phytoplasma in such hosts. The major objectives of the present work includes:In situ detection of sandal spike phytoplasma by epifluorescence microscopy and scanning electron microscopy.,Purification of sandal spike phytoplasma and production of polyclonal antibodies.,Amino acid and total protein estimation of sandal spike phytoplasma.,Immunological detection of sandal spike phytoplasma., Molecular detection of sandal spike phytoplasma.,Screening for phytoplasma in host plants of spike disease affected sandal using immunological and molecular techniques. |
Description: | Department of environmental studies, Cochin University of Science And Technology |
URI: | http://dyuthi.cusat.ac.in/purl/3421 |
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Dyuthi-T1393.pdf | (12.45Mb) |
URI: | http://dyuthi.cusat.ac.in/purl/4996 |
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Dyuthi-T2065.pdf | (39.56Mb) |
Abstract: | Nitrification is the biological oxidation of ammonium, first to nitrite and then to nitrate by two groups of aerobic, chemolithotrophic bacteria belonging to the family Nitrobacteriaceae. The biological nitrification in municipal wastewater treatment is important in those cases were ammonia removal requirement specially exist. In a trickling filter or in an activated sludge system nitrification is rate limiting and thus necessitates longer detention time. The combined carbon oxidation-nitrification processes generally have low population of nitrifiers due to a high ratio of BOD to total nitrogen in the effluent. This necessitates, separate carbon and nitrogen oxidation processes, which thus minimizes wash out ofthe nitrifiers. Therefore, a separate stage nitrification has become essential to achieve faster and efficient removal of ammonia from the wastewater. The present work deals with the development of bio reactor for nitrifying of sewage as the tertiary process so that the treated wastewater can be used for irrigation, algal culture or fish culture |
Description: | School of environmental studies, Cochin University of Science And Technology |
URI: | http://dyuthi.cusat.ac.in/purl/3444 |
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Dyuthi-T1405.pdf | (5.607Mb) |
Abstract: | In the present study the development of bioreactors for nitrifying water in closed system hatcheries of penaeid and non-penaeid prawns. This work is an attempt in this direction to cater to the needs of aquaculture industry for treatment and remediation of ammonia and nitrate in penaeid and non-penaeid hatcheries, by developing nitrifying bacteria allochthonous to the particular environment under consideration, and immobilizing them on an appropriately designed support materials configured as reactors. Ammonia toxicity is the major limiting factors in penaeid and non-penaeid hatchery systems causing lethal and sublethal effects on larvae depending on the pH values. Pressing need of the aquaculture industry to have a user friendly and economically viable technology for the removal of ammonia, which can be easily integrated to the existing hatchery designs without any major changes or modifications. Only option available now is to have biological filters through which water can be circulated for the oxidation of ammonia to nitrate through nitrite by a group of chemolithotrophs known as nitrifying bacteria. Two types of bioreactors have been designed and developed. The first category named as in situ stringed bed suspended bioreactor(SBSBR) was designed for use in the larval rearing tanks to remove ammonia and nitrite during larval rearing on a continuous basis, and the other to be used for nitrifying freshly collected seawater and spent water named as ex situ packed bed bioreactior(PBBR). On employing the two reactors together , both penaeid and non-penaeid larval rearing systems can be made a closed recirculating system at least for a season. A survey of literature revealed that the in situ stringed bed suspended reactor developed here is unique in its design, fabrication and mode of application. |
URI: | http://dyuthi.cusat.ac.in/purl/939 |
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Dyuthi-T0047.pdf | (3.513Mb) |
Abstract: | The present work deals with the development of primary cell culture and diploid cell lines from two fishes, such as Poecilia reticulata and Clarias gariepinus. The greatest difficulty experienced was the avoidance of bacterial and fungi contamination. Three types of cell cultures are commonly developed, primary cell culture, diploid cell lines and heteroploid cell lines. Primary cell culture obtained from the animal tissues that have been cultivated in vitro for the first time. They are characterized by the same chromosome number as parent tissue, cultivated in vitro for the first time, have wide range of virus susceptibility, usually not malignant, six chromatin retarded and do not grow as suspension cultures. Diploid cell lines arise from a primary cell culture at the time of subculturing. Diploid cell lines commercially used in virology are W1-38 (human embryonic lung), W1-26 (human embryonic lung) and HEX (Human embryonic kidney). Heteroploid cell lines have been subcultivated with less than 75% of the cells in the population having a diploid chromosome constitution. Tissue cultures have been extensively used in biomedical research. The main applications are in three areas, Karyological studies, Identification and study of hereditary metabolic disorders and Somatic cell genetics. Other applications are in virology and host-parasite relationships. In this study an attempt was made to preserve the ovarian tissue at low temperature in the presence of cryoprotectants so that the tissue can be retrieved at any time and a cell culture could be developed. |
URI: | http://dyuthi.cusat.ac.in/purl/106 |
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Dyuthi-T0046.pdf | (3.098Mb) |
Abstract: | Unveiling the molecular and regulatory mechanisms that prevent in vitro transformation in shrimp remains elusive in the development of continuous cell lines, with an arduous history of over 25 years (Jayesh et al., 2012). Despite presenting challenges to researchers in developing a cell line, the billion dollar aquaculture industry is under viral threat. In addition, the regulatory mechanisms that prevent in vitro transformation and carcinoma in shrimps might provide new leads for the development of anti-ageing and anti-cancer interventions in human (Vogt, 2011) and in higher vertebrates. This highlights the importance of developing shrimp cell lines, to bring out effective prophylactics against shrimp viruses and for understanding the mechanism that induce cancer and ageing in human.. Advances in molecular biology and various gene transfer technologies for immortalization of cells have resulted in the development of hundreds of cell lines from insects and mammals, but yet not a single cell line has been developed from shrimp and other marine invertebrates. With this backdrop, the research described in this thesis attempted to develop molecular tools for induced in vitro transformation in lymphoid cells from Penaeus monodon and for the development of continuous cell lines using conventional and novel technologies to address the problems at cellular and molecular level. |
Description: | National Centre for Aquatic Animal Health, School of Environmental Studies, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/3260 |
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Dyuthi-T1234.pdf | (25.33Mb) |
Description: | School of Environmental Studies, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/2182 |
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Dyuthi-T0531.pdf | (3.004Mb) |
URI: | http://dyuthi.cusat.ac.in/purl/5611 |
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Dyuthi T-2651.pdf | (46.57Mb) |
Abstract: | This thesis entitled Development of nitrifying ans photosynthetic sulfur bacteria based bioaugmentation systems for the bioremediation of ammonia and hydregen sulphide in shrimp culture. the thesis is to propose a sustainable, low cost option for the mitigation of toxic ammonia and hydrogen sulphide in shrimp culture systems. Use of ‘bioaugmentors’ as pond additives is an emerging field in aquaculture. Understanding the role of organisms involved in the ‘bioaugmentor’ will obviously help to optimize conditions for their activity.The thesis describes the use of wood powder immobilization of nitrifying consortia.Shrimp grow out systems are specialized and highly dynamic aquaculture production units which when operated under zero exchange mode require bioremediation of ammonia, nitrite nitrogen and hydrogen sulphide to protect the crop. The research conducted here is to develop an economically viable and user friendly technology for addressing the above problem. The nitrifying bacterial consortia (NBC) generated earlier (Achuthan et al., 2006) were used for developing the technology.Clear demonstration of better quality of immobilized nitrifiers generated in this study for field application. |
Description: | National Centre for Aquatic Animal Health, School of Environmental Studies, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/3076 |
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Dyuthi-T1050.pdf | (8.062Mb) |
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