Chandrasekaran, M; Rajeev Kumar, S(Elsevier, 2003)
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Abstract:
A marine Pseudomonas sp BTMS-51, immobilized by Ca-alginate gel entrapment was used for the production of extracellular Lglutaminase
under repeated batch process and continuous process employing a packed bed reactor (PBR). Immobilized cells could
produce an average of 25 U/ml of enzyme over 20 cycles of repeated batch operation and did not show any decline in production
upon reuse. The enzyme yield correlated well with the biomass content in the beads. Continuous production of the enzyme in PBR
was studied at different substrate concentrations and dilution rates. In general, the volumetric productivity increased with increased
dilution rate and substrate concentrations and the substrate conversion efficiency declined. The PBR operated under conditions
giving maximal substrate conversion efficiency gave an average yield of 21.07 U/ml and an average productivity of 13.49 U/ml/h.
The system could be operated for 120 h without any decline in productivity