Chandrasekaran, M; Nagendra, Prabhu G(Elsevier, 1997)
[+]
[-]
Abstract:
Process parameters influencing e-glutaminase production by marine Vibrio costicola in solid state fermentation
(SSF) using polystyrene as an inert support were optimised. Maximal enzyme yield (157 U/g dry
substrate) was obtained at 2% (w/w) t:glutamine, 35°C and pH 7.0 after 24 h. Maltose and potassium
dihydrogen phosphate at 1% (w/w) concentration enhanced enzyme yield by 23 and 18%, respectively, while
nitrogen sources had an inhibitory effect. Leachate with high specific activity for glutaminase (4.2 U/mg
protein) and low viscosity (0-966 Ns/m 2) was recovered from the polystyrene SSF system
Description:
Process Biochemistry, Vol. 32, No. 4, pp. 285-289, 1997
Chandrasekaran, M; Nagendra, Prabhu G(Tecpar, 1999)
[+]
[-]
Abstract:
L - Glutaminase, a therapeutically and industrially important enzyme, was produced from marine Vibrio costicola
by a novel solid state fermentation process using polystyrene beads as inert support. The new fermentation system
offered several advantages over the conventional systems, such as the yield of leachate with minimum viscosity and
high specific activity for the target product besides facilitating the easy estimation of biomass. The enzyme thus
produced was purified and characterised. It was active at physiological pH, showed high substrate specificity
towards L - glutamine and had a Km value of 7.4 x 10-2 M. It also exhibited high salt and temperature tolerance
indicating good scope for its industrial and therapeutic applications
Description:
Brazilian Archives of Biology and Technology 42(3),pp-363-368
Chandrasekaran, M; Nagendra, Prabhu G(Rapid Science Publishers, 1995)
[+]
[-]
Abstract:
Polystyrene beads, impregnated with mineral salts/glutamine medium as inert support, were used to produce
L-glutaminase from Vibrio costicola by solid-state fermentation. Maximum enzyme yield, 88 U/g substrate, was
after 36 h. Glucose at 10 g/kg enhanced the enzyme yield by 66%. The support system allowed glutaminase to
be recovered with higher specific activity and lower viscosity than when a wheat-bran system was used
Description:
World Journal of Microbiology & Biotechnology 11,683-684