Chandrasekaran, M; Nagendra, Prabhu G(Tecpar, 1999)
[+]
[-]
Abstract:
L - Glutaminase, a therapeutically and industrially important enzyme, was produced from marine Vibrio costicola
by a novel solid state fermentation process using polystyrene beads as inert support. The new fermentation system
offered several advantages over the conventional systems, such as the yield of leachate with minimum viscosity and
high specific activity for the target product besides facilitating the easy estimation of biomass. The enzyme thus
produced was purified and characterised. It was active at physiological pH, showed high substrate specificity
towards L - glutamine and had a Km value of 7.4 x 10-2 M. It also exhibited high salt and temperature tolerance
indicating good scope for its industrial and therapeutic applications
Description:
Brazilian Archives of Biology and Technology 42(3),pp-363-368