Chandrasekaran, M; Abdul Hameed, Sabu; Rajeev Kumar, S(Humana Press, 2002)
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Abstract:
L-Glutamine amidohydrolase (L-glutaminase, EC 3.5.1.2) is a therapeutically
and industrially important enzyme. Because it is a potent antileukemic
agent and a flavor-enhancing agent used in the food industry, many
researchers have focused their attention on L-glutaminase. In this article, we
report the continuous production of extracellular L-glutaminase by the
marine fungus Beauveria bassiana BTMF S-10 in a packed-bed reactor. Parameters
influencing bead production and performance under batch mode were
optimized in the order-support (Na-alginate) concentration, concentration
of CaCl2 for bead preparation, curing time of beads, spore inoculum concentration,
activation time, initial pH of enzyme production medium, temperature
of incubation, and retention time. Parameters optimized under batch
mode for L-glutaminase production were incorporated into the continuous
production studies. Beads with 12 × 108 spores/g of beads were activated in
a solution of 1% glutamine in seawater for 15 h, and the activated beads were
packed into a packed-bed reactor. Enzyme production medium (pH 9.0) was
pumped through the bed, and the effluent was collected from the top of the
column. The effect of flow rate of the medium, substrate concentration, aeration,
and bed height on continuous production of L-glutaminase was studied.
Production was monitored for 5 h in each case, and the volumetric productivity
was calculated. Under the optimized conditions for continuous production,
the reactor gave a volumetric productivity of 4.048 U/(mL·h), which indicates
that continuous production of the enzyme by Ca-alginate-immobilizedspores is well suited for B. bassiana and results in a higher yield of enzyme
within a shorter time. The results indicate the scope of utilizing immobilized
B. bassiana for continuous commercial production of L-glutaminase
Description:
Applied Biochemistry and Biotechnology,Vol. 102–103, 2002
Chandrasekaran, M; Rajeev Kumar, S; Sabu, A; Suresh, P V; Keerthi, T R(Kluwer Academic Publishers, August 6, 1999)
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Abstract:
Beauveria sp. BTMF S10 isolated from marine sediment produced extracellular L-glutaminase. Maximal L-
glutaminase yield (46.9 U/ml) was obtained in a medium supplemented with 1% (w/v) yeast extract and sorbitol,
9% (w/v) sodium chloride and 0.2% (w/v) methionine, initial pH 9.0 and at 27 °C after 108 h. This enzyme was
inducible and growth-associated.
Description:
World Journal of Microbiology & Biotechnology 15: 751±752, 1999.
Chandrasekaran, M; Suresh, P V(Elsevier, June 23, 1998)
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Abstract:
A chitinolytic fungus, Beau6eria bassiana was isolated from marine sediment and significant process parameters influencing
chitinase production in solid state fermentation using wheat bran were optimised. The organism was strongly alkalophilic and
produced maximum chitinase at pH 9·20. The NaCl and colloidal chitin requirements varied with the type of moistening medium
used. Vegetative (mycelial) inoculum was more suitable than conidial inoculum for obtaining maximal enzyme yield. The addition
of phosphate and yeast extract resulted in enhancement of chitinase yield. After optimisation, the maximum enzyme yield was
246·6 units g 1 initial dry substrate (U gIDS 1). This is the first report of the production of chitinase from a marine fungus.
Chandrasekaran, M; Suresh, P V(Rapid Science Publishers, 1998)
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Abstract:
Prawn waste, a chitinous solid waste of the shell®sh processing industry, was used as a substrate for chitinase
production by the marine fungus Beauveria bassiana BTMF S10, in a solid state fermentation (SSF) culture.
The process parameters in¯uencing SSF were optimized. A maximum chitinase yield of 248.0 units/g initial dry
substrate (U/gIDS) was obtained in a medium containing a 5:1 ratio (w/v) of prawn waste/sea water, 1% (w/w)
NaCl, 2.5% (w/w) KH2PO4, 425±600 lm substrate particle size at 27 °C, initial pH 9.5, and after 5 days of incubation.
The presence of yeast extract reduced chitinase yield. The results indicate scope for the utilization of shell®sh
processing (prawn) waste for the industrial production of chitinase by using solid state fermentation.
Description:
World Journal of Microbiology & Biotechnology, Vol 14, 1998