Padma, Nambisan; Nampoori, V P N; Vallabhan, C P G; Unni krishna PilIai, P R(1997)
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Abstract:
A comparat ive study of the effect oflaser in inducing chro mosomal
aberrat ions at 4gg nm was done in View j aba L. (faba bean)
and Allium ccpa L. (onion) with Argon ion laser (Spectra Physics Model
171). Seeds and bulbs of V.jaba and A. eepa were subjected to laser irra diation
by 4gg nm excitations from Argon ion laser source at power levels
200 and 400 mW with power densities 2.25 mW em" and 4.49 mW
em" and ditTerent exposure times (10, 20, 30 & 40 minutes). Similar to
the effect of oth er physical and chemical mutagens, laser caused a dose
dependent decrease in mitotic index and a rise in mitotic aberrations
when compared to the control. In both plant species, mutations were
observed in all stages of mitotic cell cycle. The total percentage of aberrations
was two fold higher at 400 mW than at 200 mW exposure.
Description:
GENETICS and BREEDING
Volume 28 Number3-4 pp. 27-33, 1996/1997