Increased Insulin Secretion by Muscarinic M1 and M3 Receptor Function from Rat Pancreatic Islets in vitro

Dyuthi/Manakin Repository

Increased Insulin Secretion by Muscarinic M1 and M3 Receptor Function from Rat Pancreatic Islets in vitro

Show simple item record

dc.contributor.author Renuka, T R
dc.contributor.author Remya, Robinson
dc.contributor.author Paulose,C S
dc.date.accessioned 2008-07-30T11:25:50Z
dc.date.available 2008-07-30T11:25:50Z
dc.date.issued 2005-11-02
dc.identifier.uri http://dyuthi.cusat.ac.in/purl/540
dc.description.abstract Parasympathetic system plays an important role in insulin secretion from the pancreas. Cholinergic effect on pancreatic beta cells exerts primarily through muscarinic receptors. In the present study we investigated the specific role of muscarinic M1 and M3 receptors in glucose induced insulin secretion from rat pancreatic islets in vitro. The involvement of muscarinic receptors was studied using the antagonist atropine. The role of muscarinic MI and M3 receptor subtypes was studied using subtype specific antagonists. Acetylcholine agonist, carbachol, stimulated glucose induced insulin secretion at low concentrations (10-8-10-5 M) with a maximum stimulation at 10-7 M concentration. Carbachol-stimulated insulin secretion was inhibited by atropine confirming the role of muscarinic receptors in cholinergic induced insulin secretion. Both M1 and M3 receptor antagonists blocked insulin secretion induced by carbachol. The results show that M3 receptors are functionally more prominent at 20 mM glucose concentration when compared to MI receptors. Our studies suggest that muscarinic M1 and M3 receptors function differentially regulate glucose induced insulin secretion, which has clinical significance in glucose homeostasis. en_US
dc.language.iso en en_US
dc.publisher Department of Biotechnology en_US
dc.subject Muscarinic M1 and M3 receptors en_US
dc.subject Insulin secretion en_US
dc.subject Carbachol en_US
dc.subject Atropine en_US
dc.subject Pancreatic islets en_US
dc.title Increased Insulin Secretion by Muscarinic M1 and M3 Receptor Function from Rat Pancreatic Islets in vitro en_US
dc.type Working Paper en_US


Files in this item

Files Size Format View Description
T R Renuka and others(2006)may.PDF 5.792Mb PDF View/Open pdf

This item appears in the following Collection(s)

Show simple item record

Search Dyuthi


Advanced Search

Browse

My Account