Hatha, A A M; George, M; Anjumol, A; George, G(Academic Journals, March 16, 2012)
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Abstract:
Emergence of drug resistance among pathogenic bacteria to currently available antibiotics has intensified the search for novel bioactive compounds from unexplored habitats. In the present study actinomycetes were isolated from two relatively unexplored and widely differing habitats such as mountain and wetlands and their ability to produce antibacterial substances were analyzed. Pure cultures of actinomycetes were identified by morphological and biochemical tests. Various genera of actinomycetes encountered included Nocardia, Pseudonocardia, Streptomyces, Nocardiopsis, Streptosporangium, Micromonospora, Rhodococcus, Actinosynnema, Nocardiodes, Kitasatosporia, Gordona, Intrasporangium and Streptoalloteichus. The frequency of occurrence of each genus was found to vary with sample. About 47% of wetland isolates and 33% of mountain isolates were identified as various species of Nocardia. The isolated strains differed among themselves in their ability to decompose proteins and amino acids and also in enzyme production potential. Antibiotic activities of these actinomycetes were evaluated against 12 test pathogenic bacteria by well diffusion method using agar wells in glycerol-yeast extract agar. About 95% of actinomycete isolates from wetland ecosystem and 75% of highland isolates suppressed in different degrees the growth of test pathogens. Relatively high antibacterial activity among these isolates underlined their potential as a source of novel antibiotics.
Description:
African Journal of Microbiology Research Vol. 6(10), pp. 2265-2271, 16 March, 2012
Hatha, A A M; Maya, George; Neethu, Cyriac; Aswathi, Nair(Indian Journal Of Geo Marine Sciences, June , 2011)
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Abstract:
Diversity of different groups of Bacillus and Actinomycetes in the water and sediment samples from kumarakom estuary was analysed to find out potential strains for further application. Bacillus genera was identified and grouped into five phenogroups .Phenogroups show differences in the shape of the spore,position of the spore,and swelling of the sporangium.Ability of the isolates to elaborate various hydrolytic enzymes and their ability to reduce nitrate and ferment various carbohydrate sources were also studied.
Description:
Indian Journal Of Geo Marine Sciences Vol.40(3),June 2011,PP 430-437
Hatha, A A M; Thirupathi, Suresh; Doriaraj, Sreenivasan; Sangeetha, Sreenivasan; Lakshmanaperumalsamy, P(Microbes And Enviornments, October 13, 2004)
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Abstract:
The source of samonella cross contamination in 15 retail chicken outlets in aresidual area in coimbatore city ,sourthern India was studied. Chopping boards and the butchers hands were predominant followed by knives and the weighing balance tray. Serotyping of the salmonella strains revealed that all strains were salmonella enteritis, except one which was found to be salmonella cerro.The anti bacterial activity of commonly used spices were evaluated.
Hatha, A A M; Nishamol, S; Suchitra, R; Rinoy, Varghese(Vasile Goldis University Press, 2012)
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Abstract:
TThe invention of novel antibiotics and other bioactive microbial metabolites continues to be an
important aim in new drug discovery programmes. Actinomycetes have the potential to synthesize lots of diverse
biologically vigorous secondary metabolites and in the last decades actinomycetes became the most productive
source for antibiotics. Therefore in the present study we analyze the antibacterial activity of the actinomycetes
isolated from grassland soil samples of Tropical Montane forest. A total of 33 actinomycete strains isolated were
characterized and screened for antibacterial activities using well diffusion method against six specific pathogenic
organisms. Identification of the isolates revealed that the majority of them were belonging to Streptomycetes
followed by Nocardia, Micromonospora, Pseudonocardia, Streptosporangium, Nocardiopsis and
Saccharomonospora. Among the 33 isolates, Gr1 strain showed antagonistic activity against all checked
pathogens. Nine strains showed antibacaterial activity against Listeria, Vibrio cholera, Bacillus cereus,
Staphylococcus aureus and Salmonella typhi and only 2 strains (Gr1and Gr25) showed antagonism to E. coli.
The overall percentage of activity of actinomycetes isolates against each pathogenic bacterium was also
calculated. While 63.63% of the actinomycetes were antagoinistic against Listeria, Vibrio cholerae, and Bacillus
cereus, 60.6% of them were antagonistic to Staphylococcus aureus. Very few isolates (6.06%) showed
antibacterial activity against E. coli. In general most of the actinomycetes isolates were antagonistic to grampositive
bacteria such as Listeria, Bacillus and Staphylococcus than Gram-negative bacteria Vibrio cholerae, E.
coli and Salmonella
Description:
Studia Universitatis “Vasile Goldiş”, Seria Ştiinţele Vieţii
Vol. 22, issue 3, 2012, pp. 451-455