Bright Singh, I S; Saramma, A V; Jayesh, P; Manjusha, K; Prem, Gopinath; Priyaja, P; Divya, Jose; Sreelakshmi, B(Springer, June 21, 2012)
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Abstract:
Vibrio sp. V26 isolated from mangrove
sediment showed 98 % similarity to 16S rRNA gene
of Vibrio cholerae, V. mimicus, V. albensis and uncultured
clones of Vibrio. Phenotypically also it resembled
both V. cholerae and V. mimicus.Serogrouping, virulence
associated gene profiling, hydrophobicity, and adherence
pattern clearly pointed towards the non—toxigenic
nature of Vibrio sp. V26. Purification and characterization
of the enzyme revealed that it was moderately
thermoactive, nonhemagglutinating alkaline metalloprotease
with a molecular mass of 32 kDa. The application
of alkaline protease from Vibrio sp. V26 (APV26) in sub
culturing cell lines (HEp-2, HeLa and RTG-2) and
dissociation of animal tissue (chick embryo) for primary
cell culture were investigated. The time required for
dissociation of cells as well as the viable cell yield
obtained by while administeringAPV26 and trypsin were
compared. Investigations revealed that the alkaline
protease of Vibrio sp. V26 has the potential to be used
in animal cell culture for subculturing cell lines and
dissociation of animal tissue for the development of
primary cell cultures, which has not been reported earlier
among metalloproteases of Vibrios.
Description:
Cytotechnology (2013) 65:199–212
DOI 10.1007/s10616-012-9472-z
Hatha, A A M; Mujeeb Rahiman, K M; Krishnan, K P; Saramma, A V; Saritha, G; Deepu lal(Indian Journal Of Geo Marine Sciences, August , 2013)
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Abstract:
A total of 34 yeast isolates were characterized from 4 water samples collected from Kongsfjord at Ny Alseund region of Norwegion Artic during the Indian Artic summer expedition of 2009.They were studied for the effect of tempereture and salt concentration on growth as well as for their ability to produce various hydrolytic enzymes at two different temperatures. Result showed that 5 out of 8 genera were common to all the stations. Cryptococcus was the predominant genera folowed by Trichosporan and Rhodotorula 82% of the yeast isolates were oxidative in nature and except filobasidium all the isolates used nitrate as a nitrogen source for growth. Yeast isolates from all the ststions showed growth at 4 and 20 degree centigarade. These temperatures were chosen as most of the bacterial and yeast isolates showed psychrotrop[hic nature. 94% of the yeast isolates showed growth at 2.0M and lipolytic activity were marginally less than 4.None of the isolates produced amylase enzymes when incubated at 4 and 20. The present study highlights the wide tolerence of the psychrotrophic yeast isolates to temperature and salinity as well as their potential in biotechnology
Description:
Indian Journal of Geo-marine sciences,Vol 42(4),august 2013,pp.458-465
Hatha, A A M; Shubhankar Ghosh, A; Selvam, D G; Neethu, C S; Saramma, A V(Marine Biological Association of India, January 15, 2014)
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Abstract:
Emergence of antibiotic resistance among aquaculture
pathogens has made it necessary to look into environment
friendly, effective and sustainable methods such as probiotic
and immunostimulants among others.. In the present study,
LAB were isolated from the gut of fish species namely Rastrelliger
kanagurta and analyzed for their antibacterial activity against
various fish, shrimp and human pathogens. Different LAB
species such as Lactobacillus plantarum, L. bulgaricus, L. brevis
and L. viridiscens were encountered in the gut of R. kanagurta.
Several strains showed good activity against fish, shrimp and
human pathogens. LAB from the gut of such marine species
may be developed as possible probiont for environment friendly
health management of fresh water, estuarine and marine
species currently exploited in aquaculture
Description:
J. Mar. Biol. Ass. India, 55 (2), 22-27, July-December 2013
Hatha, A A M; Mujeeb Rahiman, K M; Deepu lal, K M; Krishnan, K P; Rupesh Kumar, Sinha; Saramma, A V(Scientific publishers, 2013)
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Abstract:
Considering the extent of warming in the artic region and the resultant changes in the dynamic marine enviornments there is a need to monitor the bacterial diversity in the fjord enviornments especially in terms of cultivable bacteria. The present study reports the diversity of cultivable hetrotrophic bacteria from the water and sediment samples of kongsfjord their growth responses to important enviornmental variables and ability to produce industrially important hydrolytic enzymes.
Nithyaja, B; Jisha, V K; Tintu, R; Saramma, A V; Nampoori, V P N(Laser Physics, 2009)
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Abstract:
The growth kinetics of an aerial bacterial colony on solid agar media was studied using laser
induced fluorescence technique. Fluorescence quenching of Rhodamin B by the bacterial colony was utilized
for the study. The lag phase, log phase, and stationary phase of growth curve of bacterial colony was identified
by measuring peak fluorescence intensity of dye doped bacterial colony.