Bright Singh, I S; Rosamma, Philip; Jayaprakash, N S; Preetha, R; Sreedharan, Prathapan; Seena, Jose; Vijayan, K K(Blackwell Publishing, September 1, 2010)
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Abstract:
Persistence of the antivibrio property of the potential
antagonistic probiotics, Pseudomonas MCCB 102 and
103, at di¡erent temperatures, pH and in organic solvents
was studied. The antivibrio compound was extracted,
puri¢ed and characterized using thin-layer
chromatography, high-pressure liquid chromatography,
liquid chromatography-mass spectroscopy, UV^
Vis and nuclear magnetic resonance spectroscopy
and identi¢ed as N-methyl-1-hydroxyphenazine, a
phenazine antibiotic. The toxicity of the compound
was tested in Penaeus monodon haemocyte culture
and the IC50 valuewas found to be1.4 0.31mg L 1.
The compound was found to be bacteriostatic at
0.5mg L 1. Its stability to varying temperature, pH,
organic solvents, prolonged shelf-life and vibriostatic
nature point to its suitability for prophylatic aquaculture
application.
Bright Singh, I S; Jayaprakash, N S; Preetha, R; PhIlip, R(The Society for Applied Microbiology, July 20, 2006)
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Abstract:
Aim: To develop a new medium for enhanced production of biomass of an
aquaculture probiotic Pseudomonas MCCB 103 and its antagonistic phenazine
compound, pyocyanin.
Methods and Results: Carbon and nitrogen sources and growth factors, such as
amino acids and vitamins, were screened initially in a mineral medium for the
biomass and antagonistic compound of Pseudomonas MCCB 103. The selected
ingredients were further optimized using a full-factorial central composite
design of the response surface methodology. The medium optimized as per the
model for biomass contained mannitol (20 g l)1), glycerol (20 g l)1), sodium
chloride (5 g l)1), urea (3Æ3 g l)1) and mineral salts solution (20 ml l)1), and
the one optimized for the antagonistic compound contained mannitol (2 g l)1),
glycerol (20 g l)1), sodium chloride (5Æ1 g l)1), urea (3Æ6 g l)1) and mineral
salts solution (20 ml l)1). Subsequently, the model was validated experimentally
with a biomass increase by 19% and fivefold increase of the antagonistic
compound.
Conclusion: Significant increase in the biomass and antagonistic compound
production could be obtained in the new media.
Significance and Impact of the Study: Media formulation and optimization are
the primary steps involved in bioprocess technology, an attempt not made so
far in the production of aquaculture probiotics
Description:
Journal of Applied Microbiology 102 (2007) 1043–1051