Bright Singh, I S; Seena, Jose; Rosamma, Philip; Mohandas, A; Jayesh, P(Elsevier, December 26, 2010)
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Abstract:
Lack of shrimp cell lines has hindered the study of pollutants which adversely affects shrimp health and
its export value. In this context a primary haemocyte culture developed from Penaeus monodon was
employed for assessing the cytotoxicity and genotoxicity of two heavy metal compounds, cadmium
chloride and mercuric chloride and two organophosphate insecticides, malathion and monocrotophos.
Using MTT assay 12 h IC50 values calculated were 31.09 16.27 mM and 5.52 1.16 mM for cadmium
chloride and mercuric chloride and 59.94 52.30 mg l 1 and 186.76 77.00 mg l 1 for malathion and
monocrotophos respectively. Employing Comet assay, DNA damage inflicted by these pollutants on
haemocytes were evaluated and the pollutants induced DNA damage in >60% of the cells. The study
suggested that haemocyte culture could be used as a tool for quantifying cytotoxicity and genotoxicity of
aquaculture drugs, management chemicals and pollutants
Bright Singh, I S; Preetha, R; Jayaprakash, N S(Inter-Research, March , 2007)
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Abstract:
Synechocystis MCCB 114 and 115 were segregated as putative probionts for shrimp
larvae from a collection of 54 cyanobacterial cultures enriched from seawater. On feeding Penaeus
monodon post-larvae with the cyanobacteria, the generic diversity of the intestinal bacterial flora
could be enhanced with substantial reduction or total absence of Vibrio spp. A significant difference
(p < 0.001) in the percent survival of batches of post-larvae fed on the cyanobacterial cultures was
observed and, on repeated challenge with V. harveyi, the relative percent survival of those batches
of larvae fed on Synechocystis MCCB 114 and 115 was significantly higher. The Synechocystis
MCCB 114 and 115 cultures were found to contain high levels of protein (34 to 43%), in addition to
carotenoids