Waliaula Mola,P; Sudha, B; Paulose,C S(Department of Biotechnolgy, November , 1996)
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Abstract:
The effect of insulin on cell proliferation in vivo has been studied in hepatectomised
streptozotocin- diabetic rats. The extent of cell proliferation in sham and hepatectomized- control,
diabetic and insulin treated rats were monitored by determining DNA content and [3H]thymidine
incorporation into DNA. The kinetic parameters of thymidine kinase a regulatory enzyme for
DNA synthesis was also studied in these groups. The rate of DNA synthesis in liver of
streptozotocin -diabetic rats was significantly higher 24 hrs post-hepatectomy compared to
control and insulin treated diabetic groups. Kinetic studies of thymidine kinase revealed that there
was no change in the Michaelis -Menten constant (Km) whereas maximum velocity (Vmax) was
elevated in the diabetic hepatectomized groups compared to control and insulin treated
hepatectomized groups. Thus our study elucidates the role of insulin in thymidine kinase activity
and DNA synthesis.