Chandrasekaran, M; Nagendra, Prabhu G(Elsevier, 1997)
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Abstract:
Process parameters influencing e-glutaminase production by marine Vibrio costicola in solid state fermentation
(SSF) using polystyrene as an inert support were optimised. Maximal enzyme yield (157 U/g dry
substrate) was obtained at 2% (w/w) t:glutamine, 35°C and pH 7.0 after 24 h. Maltose and potassium
dihydrogen phosphate at 1% (w/w) concentration enhanced enzyme yield by 23 and 18%, respectively, while
nitrogen sources had an inhibitory effect. Leachate with high specific activity for glutaminase (4.2 U/mg
protein) and low viscosity (0-966 Ns/m 2) was recovered from the polystyrene SSF system
Description:
Process Biochemistry, Vol. 32, No. 4, pp. 285-289, 1997
Chandrasekaran, M; Nagendra, Prabhu G(Tecpar, 1999)
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Abstract:
L - Glutaminase, a therapeutically and industrially important enzyme, was produced from marine Vibrio costicola
by a novel solid state fermentation process using polystyrene beads as inert support. The new fermentation system
offered several advantages over the conventional systems, such as the yield of leachate with minimum viscosity and
high specific activity for the target product besides facilitating the easy estimation of biomass. The enzyme thus
produced was purified and characterised. It was active at physiological pH, showed high substrate specificity
towards L - glutamine and had a Km value of 7.4 x 10-2 M. It also exhibited high salt and temperature tolerance
indicating good scope for its industrial and therapeutic applications
Description:
Brazilian Archives of Biology and Technology 42(3),pp-363-368
Chandrasekaran, M; Suresh, P V(Rapid Science Publishers, 1998)
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Abstract:
Prawn waste, a chitinous solid waste of the shell®sh processing industry, was used as a substrate for chitinase
production by the marine fungus Beauveria bassiana BTMF S10, in a solid state fermentation (SSF) culture.
The process parameters in¯uencing SSF were optimized. A maximum chitinase yield of 248.0 units/g initial dry
substrate (U/gIDS) was obtained in a medium containing a 5:1 ratio (w/v) of prawn waste/sea water, 1% (w/w)
NaCl, 2.5% (w/w) KH2PO4, 425±600 lm substrate particle size at 27 °C, initial pH 9.5, and after 5 days of incubation.
The presence of yeast extract reduced chitinase yield. The results indicate scope for the utilization of shell®sh
processing (prawn) waste for the industrial production of chitinase by using solid state fermentation.
Description:
World Journal of Microbiology & Biotechnology, Vol 14, 1998