Chandrasekaran, M; Rajeev Kumar, S; Keerthi, T R; Sabu, A(Elsevier, September 27, 1999)
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Abstract:
Extracellular L-glutaminase production by Beau6eria sp., isolated from marine sediment, was observed during solid state
fermentation using polystyrene as an inert support. Maximal enzyme production (49.89 U:ml) occurred at pH 9.0, 27°C, in a
seawater based medium supplemented with L-glutamine (0.25% w:v) as substrate and D-glucose (0.5% w:v) as additional carbon
source, after 96 h of incubation. Enzyme production was growth associated. Results indicate scope for production of salt tolerant
L-glutaminase using this marine fungus
Chandrasekaran, M; Nagendra, Prabhu G(Rapid Science Publishers, 1995)
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Abstract:
Polystyrene beads, impregnated with mineral salts/glutamine medium as inert support, were used to produce
L-glutaminase from Vibrio costicola by solid-state fermentation. Maximum enzyme yield, 88 U/g substrate, was
after 36 h. Glucose at 10 g/kg enhanced the enzyme yield by 66%. The support system allowed glutaminase to
be recovered with higher specific activity and lower viscosity than when a wheat-bran system was used
Description:
World Journal of Microbiology & Biotechnology 11,683-684