Renuka, T R; Ani Das, V; Paulose,C S(Department of Biotechnology, March 2, 2004)
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Abstract:
Muscarinic M1 and M3 receptor changes in the brain stem during pancreatic regeneration were investigated.
Brain stem acetylcholine esterase activity decreased at the time of regeneration . Sympathetic activity also
decreased as indicated by the norepinephrine (NE) and epinephrine (EPI) content of adrenals and also in the
plasma. Muscarinic Ml and M3 receptors showed reciprocal changes in the brain stem during regeneration.
Muscairnic M1 receptor number decreased at time of regeneration without any change in the affinity. High affinity
M3 receptors showed an increase in the number. The affinity did not show any change . The number of low affinity
receptors decreased with decreased Kd at 72 hours after partial pancreatectomy. The Kd reversed to control value
with a reversal of the number of receptors to near control value . Gene expression studies also showed a similar
change in the mRNA level of Ml and M3 receptors . These alterations in the muscarinic receptors regulate
sympathetic activity and maintain glucose level during pancreatic regeneration. Central muscarinic M1 and M3
receptor subtypes functional balance is suggested to regulate sympathetic and parasympathetic activity, which in
turn control the islet cell proliferation and glucose homeostasis.
Mohanan,Valiyaveettil; Balarama Kaimal,S; Paulose, Cheramadathikudyil S(Department of Bio Technology, August 30, 2004)
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Abstract:
The present study was to investigate the rote of central 5-11T and 5-HT,:v receptor Lindin4o
and acne expression in it 'at mo(lel of pancreatic regeneration using 60" -, pancreatcutumy.
The pancreatic regeneration was evaluated by 5-HT content and 5-HT,,receptor gene
expression in the cerebral cortex (CC) and brain stem MS) of Alain opcrate,t, 7 It utd
7 (.lays panereatectomised rats. 5-11T content significantly increased in the CC' (I' 1.1)11
and 13S (P 0.05) of 72 Ii p.ntcreateetomiscd rats. Sympathetic activity was decreased as
indicated by the significantly decreased norcpiuephrine (NIi) and epinephrine (FTI) Icvcl
(1' < 0.001 and P < 0.05) in the plasma of 72 h panereateetomised rats. 5-111 ,^, receptor
density and affinity was decreased in the CC (P < 0.01) and BS (P < 0.01). These rh:)nge;
correlated with a diminished 5-IITIA receptor mRNA expression in the brain region. studied.
Our resuils suggest that the brain 5-11T through 5-HTin receptor has it funcuon:0 rule
iii 11w pi+ncreatic regcner:ttion through the sympathetic regulation.