Random genetic changes generated during in vitro culture are not desirable for plant
micropropagation and genetic transformation. RAPD markers were used to detect the variation in leaf disc callus
cultures of Jatropha curcas, maintained in Murashige and Skoog (MS) medium with different auxin and
cytokinin combinations. In total 41 scorable bands were produced with 11 primers. Out of 41 bands, 37 were
polymorphic (91.12%). The average number of polymorphic bands was 3.36 per primer. The highest similarity
(0.82) with mother plant was seen in callus maintained on MS with hormonal combination Indole butyric
acid - 0.4mg/l+ N6-benzyladenine purine - 4.0 mg/l. The callus grown on MS with hormonal combinations
IBA- 0.4mg/l+ BAP- 2.0mg/l, IBA- 0.4mg/l+ BAP- 2.5mg/l and IBA- 0.6 mg/l+ BAP- 2.0 mg/l also showed
similarity with the mother plant. Callus maintained on MS with hormonal combination IBA- 0.2mg/l+ BAP- 2.0
mg/l was found to show least similarity (0.53) with mother plant
Description:
World Journal of Agricultural Sciences 8 (6): 616-623, 2012