Ponnachan,P T C; Paulose,C S; Panikkar,K R(Department of Biotechnology, 1991)
[+]
[-]
Abstract:
Alloxan induced diabetic animal model was
sed to evaluate the potential antidiabetic effect of
ie aqueous leaf extract of Aegle marmelose. Insuinjection
and oral treatment of Aegle marmelose
of extract to the alloxan induced rats maintained
;e animal weight near to the control rats whereas
,,ere was a significant decrease in weight in alloxan
•duced rats. Insulin injection and oral treatment
the leaf extract reversed the decrease of protein
)ntent in all the organs studied except the pancas.
Glucose level was maintained near to the co-
:rol levels in the insulin injected and leaf extract
Bated alloxan diabetic rats. A significant increased
ucose tolerance was observed in animals orally
ven the leaf extract prior to the experiment. The
suits indicate that there is an increased utilization
glucose in the Aegle marmelose leaf extract treed
rats.
Ponnachan,P T C; Paulose,C S; Panikkar, K R(Department of Biotechnology, April , 1993)
[+]
[-]
Abstract:
Alloxan induced animal model was used to evaluate the potential antidiabetic effect of A . marmelose leaf
extract. The diabetic animals were given insulin injection and another group A . marmelose leaf extract orally.
It maintained the weight of the animals near to the control rats but a significant decrease in weight was noted it
diabetic animals without any treatment . The blood elucose level in 'treated animals were near to that of control
ones. Also a significantly increased glucose tolerance was observed in animals orally given the leaf extract
prior to the experiment . A significant decrease in liver glycogen ( 1.24 -.07 s!/ 100 g of wet tissue) was observed
in diabetic rats which was brought to almost the normal level (I.84- . 14 g; 100 g) with Icaf extract treatment.
Blood urea and serum cholesterol increased ( 62.66 - 3.50 and 192.67± 13.64 mg' dl) significantly in alloxxan
diabetic rats . The leaf extract treatment decreased the blood urea and serum . cholesterol (37.83 - 3.97 and
99?0±8.43 mg/dl ) to that of control ones . A similar effect was seen with insulin treatment . The results
indicate that the active principle in .4. marmelose leaf extract has similar hypoglycaemic activity to insulin
treatment.
Seema,P V; Sudha, B; Pius S,Padayatti; Asha,Abraham; Raghu,K G; Paulose,C S(Department of Biotecnology, June , 1996)
[+]
[-]
Abstract:
The functional basis of diabetes-mellitus to a certain extent,
can be elucidated by studying diabetes-induced changes
in metabolic enzymes. Malate dehydrogenase (MDH),
is an enzyme directly involved in glucose metabolism. The
kinetic parameters of MDH and its purified cytosolic isozyme,
S-MDH, have been studied in the liver of streptozotocin-
diabetic rats; also the potential of the leaf extract of A.
marmelose as an was investigated. The
Km of the liver enzyme increased significantly, in both
crude and purified preparations in the diabetic state when
compared to Lhe respective controls. Insulin as well as leaf-
•extract treatment of the diabetic rats brought about a reversal
of K. values to near normal. Vmax of purified S-MDH
was significantly higher in the diabetic state when compared
to the control. Insulin and leaf extract treatment did not
reverse this change. Since MDH is an important enzyme in
glucose metabolism, the variation in its quantitative and
qualitative nature may contribute to the pathological status
of diabetes. The fact that leaf extract of A. marmelose
was found to be as effective as insulin in restoration of blood
glucose and body weight to normal levels, the use of A.
marmelose as potential hypoglycemic agent is suggested.