Chandrasekaran, M; Nagendra, Prabhu G(Rapid Science Publishers, 1995)
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Abstract:
Polystyrene beads, impregnated with mineral salts/glutamine medium as inert support, were used to produce
L-glutaminase from Vibrio costicola by solid-state fermentation. Maximum enzyme yield, 88 U/g substrate, was
after 36 h. Glucose at 10 g/kg enhanced the enzyme yield by 66%. The support system allowed glutaminase to
be recovered with higher specific activity and lower viscosity than when a wheat-bran system was used
Description:
World Journal of Microbiology & Biotechnology 11,683-684
Somatic embryos were induced from scutellar callus of immature zygotic embryos of T aestivum cv.
Chinese Spring. Observations on precociously germinating somatic embryos revealed that: (i) In the initial
stages the coleoptile is split, exposes the shoot apex and forms a green trichomatous leafy structure.
In the germinating zygotic embryo, the coleoptile is tubular, (ii) Unlike what has been inferred earlier the
leafy structure is the coleoptile and not the scutellum, (iii) Bipolarity of the embryoid is established later
when root develops at the basal end.
Shreedevi,T T; Padayatti,P S; Kala,M S; Philip, J; Paulose,C S(Department of Biotechnology, May 25, 1994)
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Abstract:
Optical absorption characteristics of rat blood
affected by diabetes has been studied using photoacoustic
(PA) technique. PA spectrum of blood
depends on the molecular structure of haemoglobin.
The peak value ratio ylQ increases with increase in
the diabetic state. Externally added glucose to
normal blood does not show any increase in y//3 ratio
as seen in the diabetic condition . The increase in yl,8
ratio may be due to the decrease in DPG level and
the resultant shift from R -> T conformation of
majority of diabetic haemoglobin.
Paulose,C S; Athira, Babu; Anju, T R(Indian Journal of Biochemistry and Biophysics, April , 2009)
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Abstract:
Hypoxia is one of the major causes of damage to the fetal and neonatal brain and cardiac functions. in earlier studies we have reported the brain damage caused by hypoxia and resusciation with oxygen and epinephrine and have found that glucose treatment to hypoxic rats and hypoxic rats treated with oxygen shows a reversal of brain damage. during this study the findings may have clinical significance in the proper management of heart and brain functions.
Paulose,C S; Dakshinamurti,K; Subah,Packer; Newman,L Stephens(Department of Biotechnology, December 8, 1987)
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Abstract:
Pyridoxal phosphate is the coenzyme of various decarboxylases involved in the formation
of monoamine neurotransmitters such as y-aminobutyric acid , serotonin , dopamine, and norepinephrine
. Adult male Sprague-Dawley rats placed on a pyridoxine -deficient diet for 8 weeks showed
significant hypertension compared with pyridoxine -supplemented controls . Hypothalamic contents of
pyridoxal phosphate , y-aminobutyric acid, and serotonin in the pyridoxine - deficient rats were significantly
lower than those in pyridoxine -supplemented controls . Hypertension was associated with
sympathetic stimulation . Treatment of pyridoxine-deficient rats with a single dose of pyridoxine (10
mg/kg body weight) reversed the blood pressure to normal levels within 24 hours, with concomitant
restorations of hypothalamic serotonin and y-aminobutyric acid as well as the return of plasma
norepinephrine and epinephrine to normal levels . Also, pyridoxine treatment reversed the hypothalamic
hypothyroidism observed in pyridoxine -deficient rats . These results indicate an association
between pyridoxine deficiency and sympathetic stimulation leading to hypertension.
Paulose,C S; Thliveris,J A; Dakshinamurti,K; Viswanathan,M(Department of Biotechnology, 1989)
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Abstract:
We have studied testicular function in the biotin-
deficient rat biochemically and morphologically.
Serum testosterone and luteinizing hormone (LH) levels
were decreased significantly in the deficient rats. Administration
of biotin or gonadotropins to the deficient rats
reversed this decrease in serum testosterone. There was no
difference in the serum cholesterol level between the control
and biotin-deficient rats. A significant degree of sloughing
of seminiferous tubule germinal epithelium was noticed
in the biotin-deficient rat testes. Biotin treatment of biotindeficient
rats reversed this condition whereas testosterone
treatment was without any effect. The development and
maintenance of morphological and functional integrity of
the seminiferous tubules appears to require a biotin-mediated
step in addition to testosterone.
Dakshinamurti,K; Paulose,C S; Thliveris,J A; Vriend,J(Department of Biotechnology, August 15, 1984)
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Abstract:
In pyridopaminedoxine-deficient young rats hypothalamic serum TSH concentration was detected. Highly signifiserotonin
was decreased with no changes in the cant decreases in the content of pituitary TSH and in
and noradrenaline content. Serum the number of pituitary thyrotroph secretory granules
and tri-iodothyronine concentrations were were found. These results suo
mmuuchch lower in the deficient rats as compared to thyroidism of suggest that the hypocontrols.
No significant of hypothalamicp yorirdigoxinin.e -deficient young rats might bbee
difference between deficient and control groups in the
Chandrasekaran, M; Manzur Ali, P P; Rekha Mol, K R; Sapna, K; Sarita,G Bhat(Springer, March 11, 2014)
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Abstract:
Protease inhibitors can be versatile tools mainly in the fields of medicine, agriculture and
food preservative applications. Fungi have been recognized as sources of protease inhibitors,
although there are only few such reports on mushrooms. This work reports the purification and
characterization of a trypsin inhibitor from the fruiting body of edible mushroom Pleurotus
floridanus (PfTI) and its effect on the activity of microbial proteases. The protease inhibitor was
purified up to 35-fold by DEAE-Sepharose ion exchange column, trypsin-Sepharose column and
Sephadex G100 column. The isoelectric point of the inhibitor was 4.4, and its molecular mass was
calculated as 37 kDa by SDS-PAGE and 38.3 kDa by MALDI-TOF. Inhibitory activity confirmation
was by dot-blot analysis and zymographic activity staining. The specificity of the inhibitor
toward trypsin was with Ki of 1.043×10−10 M. The inhibitor was thermostable up to 90 °C with
maximal stability at 30 °C, active over a pH range of 4–10 against proteases from Aspergillus
oryzae, Bacillus licheniformis, Bacillus sp. and Bacillus amyloliquefaciens. Results indicate the
possibility of utilization of protease inhibitor from P. floridanus against serine proteases
Description:
Appl Biochem Biotechnol (2014) 173:167–178
DOI 10.1007/s12010-014-0826-1
Chandrasekaran, M; Suresh, P V(Rapid Science Publishers, 1998)
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Abstract:
Prawn waste, a chitinous solid waste of the shell®sh processing industry, was used as a substrate for chitinase
production by the marine fungus Beauveria bassiana BTMF S10, in a solid state fermentation (SSF) culture.
The process parameters in¯uencing SSF were optimized. A maximum chitinase yield of 248.0 units/g initial dry
substrate (U/gIDS) was obtained in a medium containing a 5:1 ratio (w/v) of prawn waste/sea water, 1% (w/w)
NaCl, 2.5% (w/w) KH2PO4, 425±600 lm substrate particle size at 27 °C, initial pH 9.5, and after 5 days of incubation.
The presence of yeast extract reduced chitinase yield. The results indicate scope for the utilization of shell®sh
processing (prawn) waste for the industrial production of chitinase by using solid state fermentation.
Description:
World Journal of Microbiology & Biotechnology, Vol 14, 1998
Kerala in south India grows several cash crops such as banana and
pineapple, the crop residues of which are sources of natural fibres that
can be used in hand papermaking. Kerala, however, does not have a
tradition in hand papermaking. The following is an account of an attempt
to popularize the art and craft of hand papermaking among self-help
groups as a means of self-employment and waste utilization, using fibres
extracted from agriwaste and local plants
Chandrasekaran, M; Ali, Bahkali H(Elsevier, January 11, 2013)
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Abstract:
The date palm Phoenix dactylifera has played an important role in the day-to-day life of
the people for the last 7000 years. Today worldwide production, utilization and industrialization of
dates are continuously increasing since date fruits have earned great importance in human nutrition
owing to their rich content of essential nutrients. Tons of date palm fruit wastes are discarded daily
by the date processing industries leading to environmental problems. Wastes such as date pits
represent an average of 10% of the date fruits. Thus, there is an urgent need to find suitable applications
for this waste. In spite of several studies on date palm cultivation, their utilization and scope
for utilizing date fruit in therapeutic applications, very few reviews are available and they are limited
to the chemistry and pharmacology of the date fruits and phytochemical composition, nutritional
significance and potential health benefits of date fruit consumption. In this context, in the present
review the prospects of valorization of these date fruit processing by-products and wastes’ employing
fermentation and enzyme processing technologies towards total utilization of this valuable
commodity for the production of biofuels, biopolymers, biosurfactants, organic acids, antibiotics,
industrial enzymes and other possible industrial chemicals are discussed
Description:
Saudi journal of biological sciences (2013)20,105-120