Banana waste as substrate for a-amylase production by Bacillus subtilis (CBTK 106) under solid-state fermentation

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Banana waste as substrate for a-amylase production by Bacillus subtilis (CBTK 106) under solid-state fermentation

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dc.contributor.author Chandrasekaran, M
dc.contributor.author Krishna, C
dc.date.accessioned 2014-07-23T05:03:26Z
dc.date.available 2014-07-23T05:03:26Z
dc.date.issued 1996-05-09
dc.identifier.uri http://dyuthi.cusat.ac.in/purl/4238
dc.description Appl Microbiol Biotechnol (1996) 46:106-111 en_US
dc.description.abstract Bacillus subtilis CBTK 106, isolated from banana wastes, produced high titres of a-amylase when banana fruit stalk was used as substrate in a solid-state fermentation system. The e¤ects of initial moisture content, particle size, cooking time and temperature, pH, incubation temperature, additional nutrients, inoculum size and incubation period on the production of a- amylase were characterised. A maximum yield of 5 345 000 U mg~1 min~1 was recorded when pretreated banana fruit stalk (autoclaved at 121 ¡C for 60 min) was used as substrate with 70% initial moisture content, 400 lm particle size, an initial pH of 7.0, a temperature of 35 ¡C, and additional nutrients (ammonium sulphate/sodium nitrate at 1.0%, beef extract/peptone at 0.5%, glucose/sucrose/starch/maltose at 0.1% and potassium chloride/sodium chloride at 1.0%) in the medium, with an inoculum-to-substrate ratio of 10% (v/w) for 24 h. The enzyme yield was 2.65-fold higher with banana fruit stalk medium compared to wheat bran en_US
dc.description.sponsorship Cochin University of Science and Technology en_US
dc.language.iso en en_US
dc.publisher Springer en_US
dc.title Banana waste as substrate for a-amylase production by Bacillus subtilis (CBTK 106) under solid-state fermentation en_US
dc.type Article en_US


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