dc.contributor.author |
Bright Singh, I S |
|
dc.contributor.author |
Jayaprakash, N S |
|
dc.contributor.author |
Preetha, R |
|
dc.contributor.author |
PhIlip, R |
|
dc.date.accessioned |
2014-07-15T09:15:54Z |
|
dc.date.available |
2014-07-15T09:15:54Z |
|
dc.date.issued |
2006-07-20 |
|
dc.identifier.issn |
1364-5072 |
|
dc.identifier.uri |
http://dyuthi.cusat.ac.in/purl/4036 |
|
dc.description |
Journal of Applied Microbiology 102 (2007) 1043–1051 |
en_US |
dc.description.abstract |
Aim: To develop a new medium for enhanced production of biomass of an
aquaculture probiotic Pseudomonas MCCB 103 and its antagonistic phenazine
compound, pyocyanin.
Methods and Results: Carbon and nitrogen sources and growth factors, such as
amino acids and vitamins, were screened initially in a mineral medium for the
biomass and antagonistic compound of Pseudomonas MCCB 103. The selected
ingredients were further optimized using a full-factorial central composite
design of the response surface methodology. The medium optimized as per the
model for biomass contained mannitol (20 g l)1), glycerol (20 g l)1), sodium
chloride (5 g l)1), urea (3Æ3 g l)1) and mineral salts solution (20 ml l)1), and
the one optimized for the antagonistic compound contained mannitol (2 g l)1),
glycerol (20 g l)1), sodium chloride (5Æ1 g l)1), urea (3Æ6 g l)1) and mineral
salts solution (20 ml l)1). Subsequently, the model was validated experimentally
with a biomass increase by 19% and fivefold increase of the antagonistic
compound.
Conclusion: Significant increase in the biomass and antagonistic compound
production could be obtained in the new media.
Significance and Impact of the Study: Media formulation and optimization are
the primary steps involved in bioprocess technology, an attempt not made so
far in the production of aquaculture probiotics |
en_US |
dc.description.sponsorship |
Cochin University of Science and Technology |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
The Society for Applied Microbiology |
en_US |
dc.subject |
antagonism |
en_US |
dc.subject |
aquaculture |
en_US |
dc.subject |
central composite design |
en_US |
dc.subject |
media optimization |
en_US |
dc.subject |
probiotic |
en_US |
dc.subject |
Pseudomonas |
en_US |
dc.subject |
response surface methodology |
en_US |
dc.subject |
Vibrio |
en_US |
dc.title |
Optimization of carbon and nitrogen sources and growth factors for the production of an aquaculture probiotic (Pseudomonas MCCB 103) using response surface methodology |
en_US |
dc.type |
Article |
en_US |