Bright Singh, I S; Rosamma, Philip; Anas, A; Somnath Pai, S; Douglas, W Lowman; David, L Williams; Stewart, Millen(Blackwell Publishing, July 1, 2009)
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Abstract:
E¡ect of an extraction method on the structure of
glucan and its immunostimulatory response in Fenneropenaeus
indicus was investigated. Here we extracted
alkali insoluble glucan (AIG) and alkali
soluble glucan (ASG) from a ¢lamentous fungi Acremonium
diospyri following alkali^acid hydrolysis and
the sodium hypochlorite oxidation and dimethyl
sulphoxide extraction method respectively. Structural
analysis showed that 85% of glucan in AIG was a
(1 !3)-b-D-glucan and it increased the prophenoloxidase
and reactive oxygen intermediate activity
when administered to F. indicus. On the other hand,
ASG, which contained 93% (1 !3)-a-glucan, did
not induce signi¢cant immune response in shrimp.
Here we report that the di¡erence in immunostimulatory
potential between AIG and ASG is due to the
di¡erence in the percentage of (1 !3)-b-D-glucans
present in each preparation, which varies with the
method of extraction employed. Also our observations
suggest that glucan can be used as a potential
immunostimulant to shrimp, provided it contains
(1 !3)-b-D-glucan as the major fraction.