Bright Singh, I S; Sahul Hameed, A S; Parameswaran, V; Ravi, Shukla; Thirunavukkarasu, A R; Bhonde, R R(Elsevier, January 8, 2006)
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Abstract:
A continuous cell line (SISK) from kidney of sea bass, Lates calcarifer, has been established and characterized. The cell line
was maintained in Leibovitz' L-15 supplemented with 15% fetal bovine serum. This cell line has been subcultured more than 100
times over a period of 2 years. The SISK cell line consists of predominantly of epithelial-like cells. These cells showed strong
positive for epithelial markers such as cytokeratin 19 and pancytokeratin. The cells were able to grow at temperature between 25
and 32 °C with optimum temperature of 28 °C. The growth rate of sea bass kidney cells increased as the FBS proportion increased
from 2% to 20% at 28 °C with optimum growth at the concentrations of 15% or 20% FBS. The distribution of chromosome number
was 30 to 56 with a modal peak at 48 chromosomes. Polymerase chain reaction products were obtained from SISK cells and tissues
of sea bass with primer sets of microsatellite markers of sea bass. Five fish viruses were tested on this cell line to determine its
susceptibility to these viruses and this was found to be susceptible to MABV NC1 and nodavirus, and the infection was confirmed
by RT-PCR and CPE. This suggests that the SISK cell line has good potential for the isolation of various fish viruses. This cell line
has been shown to be susceptible to bacterial extracellular products. The SISK cell line is the India's first marine fish cell line.
Bright Singh, I S; Jose, Seena; Jayesh, P(Springer, August 14, 2012)
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Abstract:
Development of continuous shrimp cell lines
for effective investigation on shrimp viruses remains elusive
with an arduous history of over 25 years. Despite
presenting challenges to researchers in developing a cell
line, the billion dollar aquaculture industry is under viral
threat. Advances in molecular biology and various gene
transfer technologies for immortalization of cells have
resulted in the development of hundreds of cell lines from
insects and mammals, but yet not a single cell line has been
developed from shrimp and other marine invertebrates.
Though improved growth and longevity of shrimp cells
in vitro could be achieved by using modified growth media
this did not make any leap to spontaneous transformation;
probably due to the fact that shrimp cells inhibited neoplastic
transformations. Oncogenic induction and immortalization
are considered as the possible ways, and an
exclusive medium for shrimp cell culture and an appropriate
mode of transformation are crucial. In this review
status of shrimp cell line development and its future orientation
are discussed
Description:
Indian J. Virol. (July–September 2012) 23(2):244–251
DOI 10.1007/s13337-012-0089-9