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Abstract:
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In the present study, the effects of 5-HT, GABA and Bone Marrow Cells
infused intranigrally to substantia nigra individually and in combinations on
unilateral rotenone infused Parkinsonism induced rats. Scatchard analysis of DA,
DA D1 and D2 receptors in the corpus striatum, cerebral cortex, cerebellum, brain
stem and hippocampus showed a significant increase in the Brain regions of
rotenone infused rat compared to control. Real Time PCR amplification of DA D1,
D2, Bax and ubiquitin carboxy-terminal hydrolase were up regulated in the brain
regions of rotenone infused rats compared to control. Gene expression studies of
-Synuclien, cGMP and Cyclic AMP response element-binding protein showed a
significant down regulation in Rotenone infused rats compared to control.
Behavioural studies were carried out to confirm the biochemical and molecular
studies.Our study demonstrated that BMC administration alone cannot reverse the
above said molecular changes occurring in PD rat. 5-HT and GABA acting
through their specific receptors in combination with bone marrow cells play a
crucial role in the functional recovery of PD rats. 5-HT, GABA and Bone marrow
cells treated PD rats showed significant reversal to control in DA receptor binding
and gene expression. 5-HT and GABA have co-mitogenic property. Proliferation
and differentiation of cells re-establishing the connections in Parkinson's disease facilitates the functional recovery. Thus, it is evident that 5-HT and GABA along
with BMC to rotenone infused rats renders protection against oxidative, related
motor and cognitive deficits which makes them clinically significant for cellbased
therapy. The BMC transformed to neurons when co-transplanted
with 5-HT and GABA which was confirmed with PKH2GL and nestin.
These newly formed neurons have functional significance in the therapeutic
recovery of Parkinson’s disease. |