Rosamma, Philip; Bright Singh, I S; Sudheer, N S(Elsevier, November 23, 2010)
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Abstract:
The objective of the study was to find out a natural way to fight white spot syndrome virus (WSSV) in cultured
shrimps, as the present scenario necessitated an organic remedy for the devastating pathogen in crustaceans.
Under this research programme seven mangrove plants were collected, identified and aqueous extracts
screened for their protective effect on the giant tiger shrimp Penaeus monodon against WSSV. The
experimental design consisted two modes of application, such as exposure of the virus to the extract and
injection challenge, and oral administration of the extract coated feed followed by oral challenge. All
experimental animals were monitored through a nested diagnostic PCR analysis. Of the seven mangrove
extracts screened aqueous extract from Ceriops tagal imparted total protection to shrimp from WSSV when
challenged by both methods. Shrimps administered with the aqueous extract from C. tagal were devoid of
virions. The HPLC fingerprint of the aqueous extracts from C. tagal showed more than 25 peaks and 7 of them
were larger and well separated. Preliminary phytochemical analysis revealed the presence of alkaloids,
flavonoids, polyphenolics, cardiac glycosides, saponins and sterols. The study indicated suitability of the
aqueous extract of C. tagal as a possible prophylaxis for WSSV infection in shrimp. This is the first report on the
anti WSSV property of the mangrove plant C. tagal