Vijayan, K K; Dr.Abdul Rasheed, T M(Cochin University Of Science And Technology, October 8, 2003)
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Abstract:
The central theme of this research concerns the study of vibrationally excited
molecules. We have used the local mode description of such vibrational states, and this
-model has now gained general acceptance. A central feature of the model is the
Wloealizafion of vibrational energy. A study of these high—energy localized states provides
example, becauseof this localization, overtone spectra, which measure the absorption of
T vibrational energy, are extremely sensitive to the properties of X-H bonds. We also use
-overtone spectra to study the conformation of molecules, i.e., the relative internal
orientation of their bonds. The thesis comprises six chapters
Description:
Department of Physics Cochin
University of Science and Technology
The present study is an attempt to find out the ralation between RNA/DNA ratio, protein,percentage growth rate and specific growth rate of prawn,Penaeus indicus with respect to Nervous system, Eyestalk and Muscle tissues during ontogenesis. We have isolated and purified a natural agglutinin in the hemolymph of P.indicus with antigenecity, agglutinating, hemolytic and antibacterial properties. The influence of growth and environmental parameters on the level of agglutinin in the hemolymph was studied. Agglutinin concentration during normal growth process was compared. The agglutinin concentration in the hemolymph was quantified through developing ELISA, which is useful in health monitoring studies of individual species. Complete amino acid composition of both the subunits of P.indicus agglutinin were analysed. P.indicus agglutinin showed similarity to those proteins having antigenecity,hemolytic and agglutinating properties.Hence, agglutinin was considered as a natural defence protein in the hemolymph of P.indicus responsible for immune surveillance. The humoral defence mechanism of agglutinin was a co-operative effort with hemocytes and complement system. The composition of isolated agglutinin of P.indicus amino acids will be helpful in the synthesis of new antibacterial analogues which can be used against disease causing organisms.
Description:
Dept. of Biotechnology, Cochin University of Science and Technology
Bijina, B; Dr.Chandrasekaran,M(Cochin University of Science & Technology, May , 2006)
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Abstract:
Protease inhibitors are one of the most important tools of nature for
regulating the proteolytic activity of their target proteases. They are
synthesized in biological systems and they play a critical role in controlling
a number of diverse physiological functions. The current investigation
focused on the isolation, purification and characterization of a novel
protease inhibitor from Moringa oleifera. The results obtained during the
course of study opens new perspectives for the utilization of protease
inhibitor from Moringa oleifera for various pharmaceutical, agricultural and
food industries. The biological and physicochemical properties exhibited by
the novel protease inhibitor from Moringa oleifera clearly testify its
suitability for the development as a drug for application in pharmaceutical
industries such as anticoagulant agent or biocontrol agent in agriculture and
even as a food preservant. There is a scope for further research on the
structure elucidation and protein engineering towards a wide range of
further applications. Detailed structure/function analysis of these proteins is
important to facilitate their use in genetic engineering for various
applications.
Description:
Department of Biotechnology,
Cocbin University of Science and Technology
Raveendran Nair, B; Dr.Joseph,Francis D(Cochin University of Science and Technology, December , 1981)
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Abstract:
Urea-formaldehyde resins find numerous
applications in adhesive, textile finishing and moulded
plastic industries. Kinetic investigations of the reactions of
urea and its related compounds with formaldehyde in
aqueous acid, alkaline and neutral media have been carried
out. A thin—layer chromatographic method was developed
for the separation and estimation of the products of these
reactions. Using this technique the various initial steps
in the reactions were analysed and the rate constants have been determined.
Description:
Department of Applied Chemistry, Cochin University of Science and Technology
Thomas,P T; George,K K(Department of Applied Economics, September , 1994)
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Abstract:
It is hoped that the present study by analysing the
dynamics of agrarian change and peasant mobility may contribute
to the on-going debate on this issue in India and other developing
countries. It may also help to throw light on the validity of some
of the theories formulated on the basis of experience in different
regions and at different times. The study is likely to be of
special importance as it covers an area which has one of the
highest densities of population. The area has also witnessed
sweeping changes in agrarian structure as a result of the most
radical land reforms among the Indian states.
Abdul Rasheed, T M; Dr.Narayanan Nampoori, V P(Cochin University of Science and Technology, September 16, 1987)
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Abstract:
Vibrational overtone spectroscopy of X-H (X=C,N,O) containing molecules is an area of recent interest. The spectroscopic studies of higher vibrational levels yield valuable informations, regarding,the molecular structure, intra- and inter-molecular interactions, radiationless transitions, intra-molecular vibrational relaxations, multiphoton excitations and chemical reactivities, which cannot be z obtained by other spectroscopic methods. This thesis presents the results of experimental investigations on the overtone spectra of some organic compounds in the liquid phase for the characterization of CH bonds. The spectra in the fifth overtone region (1fiV=6) are recorded using a dual beam thermal lens setup and the lower overtones (.AV=2-5) are recorded spectrophotometrically.The thesis is presented in six chapters.
Description:
Department of Physics,Cochin University of Science and Technology
Shibu, Eappen M; Dr.Rajappan Nair,K P(Cochin University of Science and Technology, April 4, 2003)
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Abstract:
Near-infrared spectroscopy can be a workhorse technique for materials
analysis in industries such as agriculture, pharmaceuticals, chemicals and polymers. A
near-infrared spectrum represents combination bands and overtone bands that are
harmonics of absorption frequencies in the mid-infrared. Near-infrared absorption
includes a combination-band region immediately adjacent to the mid-infrared and three
overtone regions. All four near-infrared regions contain "echoes" of the fundamental mid-infrared
absorptions. For example, vibrations in the mid-infrared due to the C-H stretches will
produce four distinct bands in each of the overtone and combination regions. As the
bands become more removed from the fundamental frequencies they become more
widely separated from their neighbors, more broadened and are dramatically reduced in
intensity. Because near-infrared bands are much less intense, more of the sample can be
used to produce a spectra and with near-infrared, sample preparation activities are
greatly reduced or eliminated so more of the sample can be utilized. In addition, long
path lengths and the ability to sample through glass in the near-infrared allows samples
to be measured in common media such as culture tubes, cuvettes and reaction bottles.
This is unlike mid-infrared where very small amounts of a sample produce a strong
spectrum; thus sample preparation techniques must be employed to limit the amount of
the sample that interacts with the beam. In the present work we describe the successful the fabrication and calibration of a linear high resolution linear spectrometer using tunable diode laser and a 36 m path length cell and meuurement of a highly resolved structure of OH group in methanol in the transition region A v =3. We then analyse the NIR spectrum of certain
aromatic molecules and study the substituent effects using local mode theory
Description:
Department of Physics, Cochin University of Science and Technology
Rajeev Kumar, S; Chandrasekaran, M(Department of Biotechnology, June , 2001)
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Abstract:
The present study is about the Pseudomonas sp. BTMS-51 isolated from the marine sediments of Cochin Coast. In the present study, it is concluded that marine bacteria are ideal candidates for immobilization using either Ca-alginate entrapment or physical adsorption on to synthetic inert supports and the process of
immobilization does not negatively influence them. Thus, Ca-alginate entrapment of the bacteria was found to be well suited for reuse of the biomass and extended
operational stability during continuous operation. Adherence of the bacterium to inertsupports was observed to be strong and it imparted minimal stress on the immobilized bacterium and allowed detachment and relocation on the supports
which enabled the formation of a dynamic equilibrium maintaining a stable cell
loading. This is particularly desirable in the industry for extended operational
stability and maintenance of consistently higher outputs. Marine Pseudomonas sp. BTMS-51 is ideal for industrial production of extra cellular L-glutaminase and immobilization on to synthetic inert support such as polyurethane foam could be an efficient technique, employing packed bed reactor for continuous production of the enzyme. Temperature and glutamine concentration had significant effects on enzyme production by cells immobilized on polyurethane foam (PUF).
Renu, S; Dr.Chandrasekaran,M(Cochin University of Science and Technology, October , 1991)
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Abstract:
L-glutaminases (L—glutamine amidohydrolase EC.3.5.l.2) is proposed as a prospective candidate for enzyme therapy cnf cancer and also as zui important additive during enzymatic digestion of shoyu koji since it could enhance glutamate content of soysauce. Commercial production of glutaminase could make possible its wide application in these areas, which would demand availability of potential sources and suitable fermentation techniques. The ‘present investigation highlighted marine environment as a potential source of efficient glutaminase producing bacteria mainly species of pseudomonas, aeromonas ,vibrio,alcaligenes, acinetobacter bacillus and planococci.Among them pseudomonas fluorescens ACMR 267 and v.cholerae ACMR 347 were chosen as the ideal strains for glutaminase production.Extracellular glutaminase fraction from all strains were in higher titres than intracellular enzymes during growth in mineral media, nutrient broth and nutrient broth added with glutamine.Glutaminase from all strains were purified employing (NH4)2SO4 fractionation followed tnr dialysis and ion exchange chromatography. The purified glutaminase from all strains were observed to be active and stable over a wide range of gfii and temperature.Optimization studies cflf environmental variables that normally influence time yiehi of glutaminase indicated that the optimal requirements of these bacteria for maximal glutaminase production remained stable irrespective of the medium, they are provided with for enzyme production. However, solid state fermentation technique was observed to
be the most suitable process for the production of Glutaminase.
Description:
Department of Applied Chemistry, Cochin
University of Science and Technology
Sabu, A; Chandrasekaran, M(Cochin University of Science and Technology, December , 1999)
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Abstract:
This study presents the L-Glutaminase Production by Marine Fungi. Enzymes are involved in all aspects of biochemical conversion from the simple enzyme or fermentation conversion to the complex techniques in genetic engineering. Enzyme industry is one among the major industries of the world and there exists a great market for enzymes in general. Food industry is recognized as the largest consumer for commercial enzymes (Lon sane and Ramakrishna, 1989). In industry, enzymes are frequently used for process improvement, for instance to enable the utilization of new types of raw materials or for improving the physical properties of a material so that it can be more easily processed. They are the focal point of biotechnological processe. The marine biosphere is one of the richest of the earth's innumerable habitats, yet is one of the least well characterized. The marine biosphere covers more than two third of the world's surface, our knowledge of marine microorganisms, in particular fungi, is still very limited (Molitoris and Schumann, 1986). The results obtained in the present study the following conclusions are drawn. Beauveria bassiana isolated form marine sediment has immense potential as an Industrial organism for production of L-glutaminase as an extracellular enzyme employing either submerged fermentnation or solid state fermentation
Nagendra, Prabhu G; Dr.Chandrasekaran,M(Cochin University of Science and Technology, June , 1996)
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Abstract:
Use of inert supports have been recommended for SSF in on ar to
overcome its inherent problems and efforts are being made to search
for newer and better materials to act as inert solid supports lidoo
et al, 1982; Zhu et al, 1994).In the present study an attempt is made to produce L-glutaminase, which is industrially and therapeutically impo rtant, from marine bacteria under solid state fermentation using natura.l.
inert and mixed substrates with a view to develop an ideal bioprocess
for its large scale production.
Description:
Microbial Technology Unit, Department of Biotechnology, Cochin University of Science and Technology
Roji, Kunnath J; Dr.Prathapachandra Kurup,M R(Cochin University Of Science And Technology, August 5, 2013)
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Abstract:
The work embodied in this thesis was carried out by the author in the Department of Applied Chemistry, CUSAT, Kochi, during the period 2008-2013. The thesis brings to light, our attempts to evaluate the coordination behavior of some compounds of interest. The biological activities of semicarbazones and their metal complexes have been an active area of research during the past years because of their significant role in naturally occurring biological systems. Tridentate NNO and ONO semicarbazone systems formed from heterocyclic and aromatic carbonyl compounds and their transition metal complexes are well-authenticated compounds in this field and their synthesis, crystal structures and spectral studies are well desirable. Hence, we decided to develop a research program aimed at the syntheses, crystal structures and spectral studies of copper complexes with halides and pseudohalides. In addition to single crystal X-ray diffraction studies, various physico-chemical methods of analysis were also used for the characterization of the complexes
Description:
Department Of Applied Chemistry
Cochin University Of Science And Technology
Annie, C F; Dr.Prathapachandra Kurup,M R(Cochin University Of Science And Technology, January 2, 2013)
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Abstract:
Semicarbazones and their transition metal complexes have been
receiving considerable attention because of their biological relevance and
applications in the field of analysis and in the field of organic NLO materials.
Their structural diversity also attracted inorganic chemists. A good deal of
work has been reported on the synthesis and structural investigation of
semicarbazones and their complexes. This is due partially to their capability
of acting as multidentate, NO, NNO, ONO and ONNO donors with the
formation of either mono or bi or polynuclear complexes. Their chemistry and
pharmacological applications have been extensively investigated. Appreciable biological applications as well as diverse stereochemistry of their metal complexes prompted us to synthesize two new tridentate ONO donor N4-phenyl semicarbazones derived from 2-hydroxy-4-methoxyacetophenone and 2-hydroxy-4-methoxybenzophenone and their transition metal complexes. These ketones were selected since they can provide a further binding site from phenolic–OH and can thus increase the denticity. Introduction of heterocyclic bases like
1,10-phenanthroline, 2,2′-bipyridine, 4,4′-dimethyl- 2,2′-bipyridine and 4-picoline
and some pseudohalides like azide and thiocyanate ion can result in mixed ligand
metal chelates with different geometries in coordination compounds In the present study, oxovanadium(IV), manganese(II), cobalt (II/III), nickel(II), copper(II) and zinc(II) complexes of 2-hydroxy-4- methoxyacetophenone-N4-phenylsemicarbazone (H2ASC) and 2-hydroxy-4-
methoxybenzophenone-N4-phenylsemicarbazone (H2BSC) were synthesized
and characterized.
Description:
Department of Applied Chemistry,
Cochin University of Science and Technology
Soorej, Basheer M; Dr.Chandrasekaran,M(Cochin University of Science and Technology, April , 2007)
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Abstract:
The present study indicate the scope for the
utilization of the marine fungus Aspergillus awamori Nagazawa BTMFW 032 for
extracellular lipase production employing submerged fermentation. To the best of
our knowledge this is the first report on lipase production by a marine fungus
employing statistical modeling towards industrial production. The characterization
of purified lipase produced by A. awamori showed stability in organic solvents,
oxidizing agent and reducing agents, I,3-regiospecificity and hydrolytic activity.
These properties make this lipase an ideal candidate for biocatalysis in organic
media for the production of novel compounds such as biodiesel and sugar fatty
esters. 91.4 % reduction in oil and grease content in ayurvedic oil by the treatment
of A. awamori lipase indicates that there is a scope for this enzyme in the treatment
of oil effluents and bioremediation. There is ample scope for further research on
the biochemistry of the enzyme, structure elucidation and enzyme engineering
towards a wide range of further applications, besides enriching scientific
knowledge on marine enzymes.
Description:
Department of Biotechnology,
Cochin University of Science and Technology