Sosamma,Cherian; Dr.George, Philip(Cochin University of Science And Technology, May , 1984)
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Abstract:
Alpha glucan phosphorylase plays a very
significant role in glycolysis. The inhibition and
activation of this enzyme have significant effect on
the rate of glycolysis. The rate of glycolysis is
also determined by the interconversion between the
active 3 and inactive Q forms of phosphorylase by
two specific enzymes called phosphorylase phosphatase
and phosphorylase kinase. The allosteric properties
and interconversion mechanism reported for
well—studied animal muscle phosphorylases do not
fall under a general pattern. Studies using
purified phosphorylase from marine sources are scanty.
Detailed studies using specialised tissues from more
marine animals are necessary to find the factors that
control the properties and activities of the enzyme.
This thesis is an attempt in this direction. The
thesis deals with a detailed study of the control of
the phosphorylase by both allosterism and
interconversion between the g and b forms from four
different aquatic animals of different habitat. Phosphorylase frm the four different animal muscles were purified either partially or completely and the kinetic and control properties were studied.
Description:
School of marine sciences, Cochin University of Science And Technology
Vijayakumar, S; Dr.George, M K(Cochin University of Science and Technology, May , 1992)
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Abstract:
The thesis deals with the results of an investigation on the "BIOCHEMICAL GENETICS OF MUGIL CEPHALUS" from Cochin, Madras and Orissa. It is presented under the following major headings: Introduction, Review of Literature, Materials and Methods, Results, Discussions, Conclusions, Recommendations, Summary and References.The introduction gives a brief account of historical and modern back ground on the stock concept in fisheries research and management, followed by the importance and potential role of biochemical genetics in the identification of natural units of fisheries management.
In the review of literature published reports relevant to biochemical genetics with special reference to that of general proteins and enzyme systems of fish populations were considered. A detailed account of the source of experimental specimens, mode of collection, transportation, sample extraction, gel preparation/gel electrophoresis, buffer systems, staining procedures of proteins/enzymes, standardization of experiments, interpretation of electrophoretic data using basic formulae etc. are given in the materials and methods section. Four important conclusions were drawn on the basis of the results of the present investigation. Three recommendations were also made on the basis of evaluation
of the results.