Bright Singh, I S; Sajeevan, T P; Rosamma, Philip(Elsevier, October 24, 2008)
[+]
[-]
Abstract:
The immunostimulatory effect of an alkali insoluble glucan extracted from marine yeast isolate Candida sake
S165 was tested in Fenneropenaeus indicus. Post larvae (PL) of F. indicus, fed glucan incorporated diet at
varying concentrations (0.05, 0.1, 0.2, 0.3, 0.4 g glucan/100 g feed) for 21 days were challenged orally with
white spot syndrome virus (WSSV). Maximum survival was observed in PL fed the 0.2% glucan incorporated
diet. Subsequently the feed incorporated with 0.2% glucan was fed to F. indicus post larvae at different
feeding intervals, i.e. daily, once every two days, once every five days, once every seven days and once every
ten days. After 40 days, the prawns were challenged orally with WSSV and post challenge survival was
recorded. Shrimp feed containing 0.2% glucan when administered once every seven days gave maximum
survival. This was supported by haematological data obtained from adult F. indicus, i.e. total haemocyte count,
phenoloxidase activity and nitroblue tetrazolium reduction (NBT). The present observation confirms the
importance of dose and frequency of administration of immunostimulants in shrimp health management
Bright Singh, I S; Sajeevan, T P; Rosamma, Philip(Elsevier, March 5, 2006)
[+]
[-]
Abstract:
The efficacy of a marine yeast Candida sake as source of immunostimulant to Indian white shrimp Fenneropenaeus indicus was
estimated. Biomass of C. sake was prepared using malt extract agar and incorporated at graded levels into a standard diet to prepare
yeast diets of varying biomass concentrations (1%, 10% and 20%). F. indicus were fed on these diets for a period of 28 days and
challenged orally with white spot syndrome virus (WSSV) and immune parameters such as total haemocyte count, phenoloxidase
and nitroblue tetrazolium reduction (NBT) were determined. Ten per cent C. sake in the diet was found to support an optimum
immune response in the animals in general and their enhancement could be observed on the second and third day following
challenge with the virus. The study has demonstrated that marine yeast C. sake at 10% in diet (w/w) may be used as an effective
source of immunostimulants in F. indicus