Madhavan Nanpoothiri, K; Dr.Ashok, Pandey(Cochin University Of Science And Technology, July 8, 1997)
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Abstract:
The thesis comprises a set of experiments mainly focused on the
improvement of L-glutamic acid fennentation. Much attention has been given to
use of locally available raw materials, culturing the organism on inert solid
substrates and also immobilization of the bacterial cells from the view point of long
term utilization of biocatalyst and continuous operation of the stabilized system.
Studies were also carried out for the down stream processing for the extraction and
purification of L-glutamic acid. An attempt was made to study the morphological
features of the microorganism including the cell premeability. In relation with the
accumulation of glutamic acid within the cells an approach was made to study the
behaviour of the Brevibacterium cells when they are exposed to hyper osmotic
environment. Attempts were also made to study the requirement of iron and
production of siderophores by this microbial strain. The search for a suitable
nitrogen source for glutamate fermentation ended with a promising result that they
got a potent urease activity and it can be utilized for many biotransfonnation
studies.
The entire thesis is presented in three sections, viz. introductory section,
experimental section and the concluding section
Description:
Department Of Biotechnology,Cochin University Of Science And Technology