Now showing items 1-5 of 5
| Abstract: | A Pseudomonas sp PS-102 recovered from Muttukkadu brackish water lagoon, situated south of Chennai, showed significant activity against a number of shrimp pathogenic vibrios. Out of the 112 isolates of bacterial pathogens comprising Vibrio harveyi, V. vulnificus, V. parahaemolyticus, V. alginolyticus, V. fluvialis, and Aeromonas spp, 73% were inhibited in vitro by the cell-free culture supernatant of Pseudomonas sp PS-102 isolate. The organism produced yellowish fluorescent pigment on King's B medium, hydrolysed starch and protein, and produced 36.4% siderophore units by CAS assay and 32 μM of catechol siderophores as estimated by Arnow's assay. The PS-102 isolate showed wide ranging environmental tolerance with, temperatures from 25 to 40 °C, pH from 6 to 8, salinity from 0 to 36 ppt, while the antagonistic activity peaked in cultures grown at 30 °C, pH 8.0 and at 5 ppt saline conditions. The antagonistic activity of the culture supernatant was evident even at 30% v / v dilution against V. harveyi. The preliminary studies on the nature of the antibacterial action indicated that the antagonistic principle as heat stable and resistant to proteolytic, lipolytic and amylolytic enzymes. Pseudomonas sp PS 102 was found to be safe to shrimp when PL-9 stage were challenged at 107 CFU ml−1 and by intramuscular injection into of ∼5 g sub-adults shrimp at 105 to 108 CFU. Further, its safety in a mammalian system, tested by its pathogenicity to mice, was also determined and its LD50 to BALB/c mice was found to be 109 CFU. The results of this study indicated that the organism Pseudomonas sp PS 102 could be employed as a potential probiont in shrimp and prawn aquaculture systems for management and control of bacterial infections |
| Description: | Aquaculture 251 (2006) 192–200 |
| URI: | http://dyuthi.cusat.ac.in/purl/4023 |
| Files | Size |
|---|---|
| A brackishwater ... enaeid rearing systems.pdf | (152.9Kb) |
| Abstract: | Persistence of the antivibrio property of the potential antagonistic probiotics, Pseudomonas MCCB 102 and 103, at di¡erent temperatures, pH and in organic solvents was studied. The antivibrio compound was extracted, puri¢ed and characterized using thin-layer chromatography, high-pressure liquid chromatography, liquid chromatography-mass spectroscopy, UV^ Vis and nuclear magnetic resonance spectroscopy and identi¢ed as N-methyl-1-hydroxyphenazine, a phenazine antibiotic. The toxicity of the compound was tested in Penaeus monodon haemocyte culture and the IC50 valuewas found to be1.4 0.31mg L 1. The compound was found to be bacteriostatic at 0.5mg L 1. Its stability to varying temperature, pH, organic solvents, prolonged shelf-life and vibriostatic nature point to its suitability for prophylatic aquaculture application. |
| Description: | Aquaculture Research, 2010, 41, 1452^1461 doi:10.1111/j.1365-2109.2009.02436.x |
| URI: | http://dyuthi.cusat.ac.in/purl/4047 |
| Files | Size |
|---|---|
| An inhibitory c ... ness on Vibrio harveyi.pdf | (371.8Kb) |
| Abstract: | The central theme of this research concerns the study of vibrationally excited molecules. We have used the local mode description of such vibrational states, and this -model has now gained general acceptance. A central feature of the model is the Wloealizafion of vibrational energy. A study of these high—energy localized states provides example, becauseof this localization, overtone spectra, which measure the absorption of T vibrational energy, are extremely sensitive to the properties of X-H bonds. We also use -overtone spectra to study the conformation of molecules, i.e., the relative internal orientation of their bonds. The thesis comprises six chapters |
| Description: | Department of Physics Cochin University of Science and Technology |
| URI: | http://dyuthi.cusat.ac.in/purl/3812 |
| Files | Size |
|---|---|
| Dyuthi-T1743.pdf | (7.080Mb) |
| Abstract: | Penaeid prawns form the most economically significant group in the marine and brackishwater fishery resources of India. This particular group contributes about 62% of the total prawn landings of the country. At present prawns have assumed an important place especially as a commodity supporting an export trade of sizable magnitude. Considerable interest has been shown in the last decade to increase prawn production through various culture practices, mainly due to the high demand for good quality prawns for export coupled with the stagnant and even depleting nature of marine catches. Available informations suggest that among the 15 species of shrimps and prawns occurring in Indian waters, which are deemed suitable for aquaculture, the Indian white prawn §.indicus is identified as one of the most important commercial species. Considering the increasing importance as an accepted species for prawn culture, £.indicus was selected for the present study. In the life history of prawns, moulting is an important event, which enables the animal to achieve growth. This dynamic physiological event continues through out the life span ofthe prawn, linking almost all biological activities with this process. Hence, a good knowledge pertaining to the physiology of moulting is imperative to understand the growth process. This knowledge will be of great use in the scientific prawn farming, so as to achieve high prawn production. |
| Description: | School of marine sciences, Central institute of fisheries technology, Cochin University of Science And Technology |
| URI: | http://dyuthi.cusat.ac.in/purl/3437 |
| Files | Size |
|---|---|
| Dyuthi-T1403.pdf | (6.680Mb) |
| URI: | http://dyuthi.cusat.ac.in/purl/1237 |
| Files | Size |
|---|---|
| Vijayan K K 1989.PDF | (679.7Kb) |
Now showing items 1-5 of 5
Dyuthi Digital Repository Copyright © 2007-2011 Cochin University of Science and Technology. Items in Dyuthi are protected by copyright, with all rights reserved, unless otherwise indicated.