Hatha, A A M; Shrishti, Kumar; Christi, K S(Rev. Biol. Trop., May 7, 2007)
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Abstract:
The diversity and load of heterotrophic bacteria and fungi associated with the mangrove soil from
Suva, Fiji Islands, was determined by using the plate count method. The ability of the bacterial isolates to produce
various hydrolytic enzymes such as amylase, gelatinase and lipase were determined using the plate assay.
The heterotrophic bacterial load was considerably higher than the fungal load. There was a predominance of
the gram positive genus, Bacillus. Other genera encountered included Staphylococcus, Micrococcus, Listeria
and Vibrio. Their effectiveness on the degradation of commercial polythene carry bags made of high density
polyethylene (HDPE) and low density polyethylene (LDPE) was studied over a period of eight weeks in the
laboratory. Biodegradation was measured in terms of mean weight loss, which was nearly 5 % after a period of
eight weeks. There was a significant increase in the bacterial load of the soil attached to class 2 (HDPE) polythene.
After eight weeks of submergence in mangrove soil, soil attached to class 1 and class 3 polythene mostly
had Bacillus (Staphylococcus predominated in class 2 polythene). While most of the isolates were capable of
producing hydrolytic enzymes such as amylase and gelatinase, lipolytic activity was low. Class 2 HDPE suffered
the greatest biodegradation.
Hatha, A A M; Maya, George; Neethu, Cyriac; Aswathi, Nair(Indian Journal Of Geo Marine Sciences, June , 2011)
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Abstract:
Diversity of different groups of Bacillus and Actinomycetes in the water and sediment samples from kumarakom estuary was analysed to find out potential strains for further application. Bacillus genera was identified and grouped into five phenogroups .Phenogroups show differences in the shape of the spore,position of the spore,and swelling of the sporangium.Ability of the isolates to elaborate various hydrolytic enzymes and their ability to reduce nitrate and ferment various carbohydrate sources were also studied.
Description:
Indian Journal Of Geo Marine Sciences Vol.40(3),June 2011,PP 430-437
Chandrasekaran, M; Dr.Lakshmanaperumalsamy, P; Dr.Chandramohan, D(Cochin University of Science and Technology, April , 1985)
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Abstract:
The aim of the present investigation is to build up the knowledge on the role of commensal bacteria present on the prawns during storage at various temperatures. The study Evaluates the nature of spoilage of prawns during storage at three different temperatures (28:2OC, 4°C and -18°C) by organoleptic assessment, accumulation of trim ethylamine, ammonia content, changes in the flesh pH and total heterotrophic bacterial population at various time intervals and to find out the changes in the proximate composition (protein, carbohydrate, lipid, ash and moisture) of the prawns during storage at various temperatures by estimating the contents at different time intervals along with spoilage assessment. The researcher studies the occurrence and role of various bacterial genera which form the component of spoilage flora during storage and determines the distribution of various hydrolytic enzyme producing bacteria by evaluating their ability to produce enzymes such as caseinase, gelatinase, amylase, lipase and urease. to assess the spoilage potential of the bacteria by testing their ability to reduce trimethylamine oxide (TMAO) to trimethylamine (TMA) and to produce odour in flesh broth and halos in flesh agar media.The researcher also gives stress on the growth kinetics of selected potential spoilers by growing_them in different media and to assess the effect of sodium chloride concentrations, temperature and pH on their growth, survival and. generation time.
Description:
School of Marine Sciences, Division of marine biology, microbiology and biochemistry, Cochin University of Science and Technology