Hari Sankar, B; Dr Ramaiah(Cochin University of Science and Technology, January 8, 2015)
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Abstract:
Development of molecular probes for the selective and sensitive detection of
analytes has been an active area of research because such effective molecules can have
biological and medicinal applications. Of the various probes reported, the fluorescence
based systems have attracted much attention owing to their high sensitivity, fast
response time, non–invasiveness and technical simplicity. However, the quantification of
a target analyte using the fluorescent probe that acts through a single emission response
(either increase or decrease in the fluorescence intensity) can have disadvantages, when
compared to the systems that show either dual emission or ratiometric changes. In the
case of the systems that exhibit ratiometric changes, the ratio of the fluorescence
intensity of the two different wavelengths can be utilized for the quantification of the
analyte of interest. Such an observation will have an effective internal referencing that
greatly increases the sensitivity of the detection. In this context, it was our objective to
develop functional organic systems and to explore their potential as sensitive
fluorescent probes for the recognition of anions, cations and biomolecules. The present
thesis has been divided into four chapters and of which the first chapter gives an
overview on the organic fluorescent probes, with a particular emphasis on their
mechanism of molecular recognition. In addition, the specific objectives of the present
thesis were also briefly described at the end of this chapter.