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| Abstract: | An attempt has been made in this study to screen some fish muscle enzymes to assess their potential worth in testing the degree of freshness of fish. A problem with routine enzyme activity determinations is the complexity of the method of enzyme assay. Hence, in the present study as far as possible simple assay techniques were adopted. Several species were screened to assess the possibility of employing this procedure on a large scale. It is hoped that findings of this study will lead to the development of meaningful criteria in testing the freshness of fish. This thesis has been divided into five chapters |
| Description: | Division of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University ofScience and Technology |
| URI: | http://dyuthi.cusat.ac.in/purl/3590 |
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| Dyuthi-T1530.pdf | (4.119Mb) |
| Abstract: | Alpha glucan phosphorylase plays a very significant role in glycolysis. The inhibition and activation of this enzyme have significant effect on the rate of glycolysis. The rate of glycolysis is also determined by the interconversion between the active 3 and inactive Q forms of phosphorylase by two specific enzymes called phosphorylase phosphatase and phosphorylase kinase. The allosteric properties and interconversion mechanism reported for well—studied animal muscle phosphorylases do not fall under a general pattern. Studies using purified phosphorylase from marine sources are scanty. Detailed studies using specialised tissues from more marine animals are necessary to find the factors that control the properties and activities of the enzyme. This thesis is an attempt in this direction. The thesis deals with a detailed study of the control of the phosphorylase by both allosterism and interconversion between the g and b forms from four different aquatic animals of different habitat. Phosphorylase frm the four different animal muscles were purified either partially or completely and the kinetic and control properties were studied. |
| Description: | School of marine sciences, Cochin University of Science And Technology |
| URI: | http://dyuthi.cusat.ac.in/purl/3412 |
| Files | Size |
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| Dyuthi-T1311.pdf | (6.313Mb) |
| Abstract: | ac-qlncnn phosphorylsse is en ilportent enzyme in glycoiysis. It is the first used knows to exhibit ellosteric properties and lance its inhibition end ectivetion have significant effect on the rete ot qlycolysis. The thesis deals with 11 detailed study of the structure. inhibition and control or this snlrlls from rabbit uncle and troll e merino eninelo ‘the thesis is divided into two parts. Port 1 deals with studies on rabbit uncle glycogen phospherylese. After e review of the relevant literetnre (Chapter 1) the inhibition and chancel sodiiicetion studies on rabbit ensyle ere discussed in chepters 2 to 5. Chapter 6. gives the methods used for the study |
| Description: | School of Marine Sciences, Cochin University of Science and Technology |
| URI: | http://dyuthi.cusat.ac.in/purl/3652 |
| Files | Size |
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| Dyuthi-T1626.pdf | (10.43Mb) |
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