Chandrasekaran, M; Madhu, K M; Beena, P S(Springer, 2009)
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Abstract:
A potential fungal strain producing extracellular β-glucosidase enzyme was isolated from sea water and identified as ^ëéÉêJ
Öáääìë=ëóÇçïáá BTMFS 55 by a molecular approach based on 28S rDNA sequence homology which showed 93% identity
with already reported sequences of ^ëéÉêÖáääìë=ëóÇçïáá in the GenBank. A sequential optimization strategy was used to enhance
the production of β-glucosidase under solid state fermentation (SSF) with wheat bran (WB) as the growth medium.
The two-level Plackett-Burman (PB) design was implemented to screen medium components that influence β-glucosidase
production and among the 11 variables, moisture content, inoculums, and peptone were identified as the most significant
factors for β-glucosidase production. The enzyme was purified by (NH4)2SO4 precipitation followed by ion exchange chromatography
on DEAE sepharose. The enzyme was a monomeric protein with a molecular weight of ~95 kDa as determined
by SDS-PAGE. It was optimally active at pH 5.0 and 50°C. It showed high affinity towards éNPG and enzyme has a hã and
sã~ñ of 0.67 mM and 83.3 U/mL, respectively. The enzyme was tolerant to glucose inhibition with a há of 17 mM. Low concentration
of alcohols (10%), especially ethanol, could activate the enzyme. A considerable level of ethanol could produce from
wheat bran and rice straw after 48 and 24 h, respectively, with the help of p~ÅÅÜ~êçãóÅÉë=ÅÉêÉîáëá~É in presence of cellulase
and the purified β-glucosidase of ^ëéÉêÖáääìë=ëóÇçïáá BTMFS 55.
Description:
Biotechnology and Bioprocess Engineering 2009, 14: 457-466
DOI/10.1007/s12257-008-0116-2