Bijina, B; Dr.Chandrasekaran,M(Cochin University of Science & Technology, May , 2006)
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Abstract:
Protease inhibitors are one of the most important tools of nature for
regulating the proteolytic activity of their target proteases. They are
synthesized in biological systems and they play a critical role in controlling
a number of diverse physiological functions. The current investigation
focused on the isolation, purification and characterization of a novel
protease inhibitor from Moringa oleifera. The results obtained during the
course of study opens new perspectives for the utilization of protease
inhibitor from Moringa oleifera for various pharmaceutical, agricultural and
food industries. The biological and physicochemical properties exhibited by
the novel protease inhibitor from Moringa oleifera clearly testify its
suitability for the development as a drug for application in pharmaceutical
industries such as anticoagulant agent or biocontrol agent in agriculture and
even as a food preservant. There is a scope for further research on the
structure elucidation and protein engineering towards a wide range of
further applications. Detailed structure/function analysis of these proteins is
important to facilitate their use in genetic engineering for various
applications.
Description:
Department of Biotechnology,
Cocbin University of Science and Technology
Sona, A; Dr. Mukundan, M K(Central Institute of Fisheries Technology, July , 2004)
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Abstract:
In the attempt to find out catalytic potency and properties of the endoglucanase
of green mussel, it could be highlighted that the enzyme is efficient in degrading
carboxymethylcellulose to reducing sugars. The immobilized enzyme will find
applications in the food industry, paper and pulp industry, wood preservation,
alcohol and pharmaceutical industry.The purification method employed i.e. Sephadex G100 chromatography
employing affinity and exclusion principles simplify the purification procedure.Addition of Mg2+ and Co2+ at 10mM concentrations enhances endoglucanase
activity of green mussel.The immobilized endoglucanase can be used for deinking mixed office waste
paper. The endoglucanase if supplemented with exoglucanase and B-glucosidase
under appropriate conditions would help in the recycling of paper.