Jayesh, P; Dr.Bright Singh,I S(Cochin University of Science and Technology, September , 2012)
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Abstract:
Unveiling the molecular and regulatory mechanisms that prevent in vitro
transformation in shrimp remains elusive in the development of continuous cell
lines, with an arduous history of over 25 years (Jayesh et al., 2012). Despite
presenting challenges to researchers in developing a cell line, the billion dollar
aquaculture industry is under viral threat. In addition, the regulatory mechanisms
that prevent in vitro transformation and carcinoma in shrimps might provide new
leads for the development of anti-ageing and anti-cancer interventions in human
(Vogt, 2011) and in higher vertebrates. This highlights the importance of
developing shrimp cell lines, to bring out effective prophylactics against shrimp
viruses and for understanding the mechanism that induce cancer and ageing in
human.. Advances in molecular biology and various gene transfer technologies for
immortalization of cells have resulted in the development of hundreds of cell lines
from insects and mammals, but yet not a single cell line has been developed from
shrimp and other marine invertebrates. With this backdrop, the research described
in this thesis attempted to develop molecular tools for induced in vitro
transformation in lymphoid cells from Penaeus monodon and for the development
of continuous cell lines using conventional and novel technologies to address the
problems at cellular and molecular level.
Description:
National Centre for Aquatic Animal
Health, School of Environmental Studies, Cochin University of Science and
Technology
Bright Singh, I S; Jose, S; Jayesh, P; Sudheer, N S; Poulose, G; Mohandas, A; PhIlip, R(Blackwell Publishing, May 1, 2012)
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Abstract:
Shrimp cell lines are yet to be reported and this
restricts the prospects of investigating the associated
viral pathogens, especially white spot syndrome
virus (WSSV). In this context, development of
primary cell cultures from lymphoid organs was
standardized. Poly-l-lysine-coated culture vessels
enhanced growth of lymphoid cells, while the
application of vertebrate growth factors did not,
except insulin-like growth factor-1 (IGF-1). Susceptibility
of the lymphoid cells to WSSV was
confirmed by immunofluoresence assay using
monoclonal antibody against the 28 kDa envelope
protein of WSSV. Expression of viral and immunerelated
genes in WSSV-infected lymphoid cultures
could be demonstrated by RT-PCR. This emphasizes
the utility of lymphoid primary cell culture as a
platform for research in virus–cell interaction, virus
morphogenesis, up and downregulation of shrimp
immune-related genes, and also for the discovery of
novel drugs to combat WSSV in shrimp culture
Description:
Journal of Fish Diseases 2012, 35, 321–334 doi:10.1111/j.1365-2761.2012.01348.x