Chandrasekaran, M; Madhu, K M; Beena, P S(Springer, 2009)
[+]
[-]
Abstract:
A potential fungal strain producing extracellular β-glucosidase enzyme was isolated from sea water and identified as ^ëéÉêJ
Öáääìë=ëóÇçïáá BTMFS 55 by a molecular approach based on 28S rDNA sequence homology which showed 93% identity
with already reported sequences of ^ëéÉêÖáääìë=ëóÇçïáá in the GenBank. A sequential optimization strategy was used to enhance
the production of β-glucosidase under solid state fermentation (SSF) with wheat bran (WB) as the growth medium.
The two-level Plackett-Burman (PB) design was implemented to screen medium components that influence β-glucosidase
production and among the 11 variables, moisture content, inoculums, and peptone were identified as the most significant
factors for β-glucosidase production. The enzyme was purified by (NH4)2SO4 precipitation followed by ion exchange chromatography
on DEAE sepharose. The enzyme was a monomeric protein with a molecular weight of ~95 kDa as determined
by SDS-PAGE. It was optimally active at pH 5.0 and 50°C. It showed high affinity towards éNPG and enzyme has a hã and
sã~ñ of 0.67 mM and 83.3 U/mL, respectively. The enzyme was tolerant to glucose inhibition with a há of 17 mM. Low concentration
of alcohols (10%), especially ethanol, could activate the enzyme. A considerable level of ethanol could produce from
wheat bran and rice straw after 48 and 24 h, respectively, with the help of p~ÅÅÜ~êçãóÅÉë=ÅÉêÉîáëá~É in presence of cellulase
and the purified β-glucosidase of ^ëéÉêÖáääìë=ëóÇçïáá BTMFS 55.
Description:
Biotechnology and Bioprocess Engineering 2009, 14: 457-466
DOI/10.1007/s12257-008-0116-2
Bright Singh, I S; Rosamma, Philip; Jayesh, P(Springer, February 25, 2014)
[+]
[-]
Abstract:
Development of continuous cell lines from
shrimp is essential to investigate viral pathogens.
Unfortunately, there is no valid cell line developed
from crustaceans in general and shrimps in particular to
address this issue. Lack of information on the requirements
of cells in vitro limits the success of developing a
cell line, where the microenvironment of a cell culture,
provided by the growthmedium, is of prime importance.
Screening and optimization of growth medium components
based on statistical experimental designs have
been widely used for improving the efficacy of cell
culture media. Accordingly, we applied Plackett–Burman
design and response surface methodology to study
multifactorial interactions between the growth factors in
shrimp cell culture medium and to identify the most
important ones for growth of lymphoid cell culture from
Penaeus monodon. The statistical screening and
optimization indicated that insulin like growth factor-I
(IGF-I) and insulin like growth factor-II (IGF-II) at
concentrations of 100 and 150 ng ml-1, respectively,
could significantly influence the metabolic activity and
DNA synthesis of the lymphoid cells. An increase of
53 % metabolic activity and 24.8 % DNA synthesis
could be obtained, which suggested that IGF-I and IGFII
had critical roles in metabolic activity and DNA
synthesis of shrimp lymphoid cells