Ani Das, V; Paulose,C S(Department of Biotechnology, Faculty of Science, August , 2000)
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Abstract:
In the present study, the changes in the brain EPI (Epinephrine), adrenergic receptors and the receptor gene expression were investigated during pancreatic regeneration and insulin secretion. The changes in the pancreatic islet EPI and adrenergic receptors were also studied in the pancreatectomised rats. The regulatory function of EPI in association with Epidermal growth factor (EGF) and glucose were investigated in rat islet cultures. In vitro studies were carried out using antagonists for adrenergic receptor subtypes to see their involvement in the islet DNA synthesis. The mechanism by which the peripheral EPI regulate insulin secretion was also investigated by studying the nuclear binding proteins in the pancreatic islets during pancreatic regeneration and diabetes. The study reveals that EPI can regulate the pancreatic islet cell proliferation by controlling the insulin synthesis and secretion. The brain adrenergic receptor gene expression and functional correlation regulate the pancreatic adrenergic receptors. The functional balance of α and β-adrenergic receptors controls the insulin secretion and pancreatic β-cell proliferation, which will have immense clinical significance in the treatment of Diabetes mellitus.
Ani Das, V; Savitha,B; Paulose,C S(Department of Biotechnology, April 4, 2006)
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Abstract:
purpose of this study was to investigate the
role of brain al-adrenergic receptor binding in the rat model
of pancreatic regeneration using 60-70% pancre:dectorny.
The a, -adrenergic receptors kinetics was studied in the
cerebral cor:cx and brain stem of sham operated . 72 It pan-
crea(ectoinised and 7 days pancreatectomised rats. Scar
chard analysis with I `I I lprazocin in cerebral cartes and brain
stein showed a significant decrease (/' < 0.01). (P < 0.05)
in maximal binding ( 1),,,,,) with it significant decrease
(P < 0.001 ), ( P < 0.01) in the K,,in 72 It pancreatecto-
raised rats compared with sham , respectively . Competition
analysis in cerebral cortex and brain stem showed it shift in
affinity during pancreatic regeneration . The sympathetic
activity was decreased as indicated by the significantly de-
increased norepinephrine level in the plasma (P < 0.001),
cerebral cortex (P < 0.01) and brain stem (P < 0.001) of
72 h pancreatectomised rats compared to sham . Thus, from
our results it is suggested that the central a, -adrenergic
receptors have a functional role in the pancreatic regenera-
Lion mediated through the sympathetic pathway.