Renuka, T R; Ani Das, V; Paulose,C S(Department of Biotechnology, March 2, 2004)
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Abstract:
Muscarinic M1 and M3 receptor changes in the brain stem during pancreatic regeneration were investigated.
Brain stem acetylcholine esterase activity decreased at the time of regeneration . Sympathetic activity also
decreased as indicated by the norepinephrine (NE) and epinephrine (EPI) content of adrenals and also in the
plasma. Muscarinic Ml and M3 receptors showed reciprocal changes in the brain stem during regeneration.
Muscairnic M1 receptor number decreased at time of regeneration without any change in the affinity. High affinity
M3 receptors showed an increase in the number. The affinity did not show any change . The number of low affinity
receptors decreased with decreased Kd at 72 hours after partial pancreatectomy. The Kd reversed to control value
with a reversal of the number of receptors to near control value . Gene expression studies also showed a similar
change in the mRNA level of Ml and M3 receptors . These alterations in the muscarinic receptors regulate
sympathetic activity and maintain glucose level during pancreatic regeneration. Central muscarinic M1 and M3
receptor subtypes functional balance is suggested to regulate sympathetic and parasympathetic activity, which in
turn control the islet cell proliferation and glucose homeostasis.
Anju, T R; Dr. Paulose, C S(Cochin University of Science and Technology, September , 2010)
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Abstract:
The present study was designed to investigate the protective effect of
glucose, oxygen and epinephrine resuscitation on impairment in the functional role
of GABAergic, serotonergic, muscarinic receptors, PLC, BAX, SOD, CAT and
GPx expression in the brain regions of hypoxia induced neonatal rats. Also, the
role of hormones - Triiodothyronine (T3) and insulin, second messengers –
cAMP, cGMP and IP3 and transcription factors – HIF and CREB in the regulation
of neonatal hypoxia and its resuscitation methods were studied. Behavioural
studies were conducted to evaluate the motor function and cognitive deficit in one
month old control and experimental rats. The efficient and timely supplementation
of glucose plays a crucial role in correcting the molecular changes due to hypoxia,
oxygen and epinephrine. The sequence of glucose, epinephrine and oxygen
administration at the molecular level is an important aspect of the study. The
additive neuronal damage effect due to oxygen and epinephrine treatment is
another important observation. The corrective measures by initial supply of
glucose to hypoxic neonatal rats showed from the molecular study when brought
to practice will lead to healthy intellectual capacity during the later developmental
stages, which has immense clinical significance in neonatal care.
Description:
Department of Biotechnology,
Cochin University of Science and Technology
Reas Khan, S; Dr. Paulose, C S(Cochin University of Science and Technology, April , 2007)
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Abstract:
The present work is to understand the alterations of total muscarinic.
muscarinic MI and glutamate receptors in the brain regions of pilocarpine induced
epileptic rats. The work focuses on the evaluation of the anti epileptic activity of
extracts of Bacopa monnieri, Bacoside A and Carbamazepine in vivo. The
molecular changes in the muscarinic M I receptors in the pre- and post-treated
epileptic model with Bacopa monnieri, Bacoside A and Carbamazepine were also
studied. These studies will help us to elucidate the functional role of muscarinic
and glutamate receptors in epilepsy.
Description:
Department of Biotechnology, Cochin University of
Science and Technology