Chandrasekaran, M; Rajeev Kumar, S(Elsevier, 2003)
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Abstract:
A marine Pseudomonas sp BTMS-51, immobilized by Ca-alginate gel entrapment was used for the production of extracellular Lglutaminase
under repeated batch process and continuous process employing a packed bed reactor (PBR). Immobilized cells could
produce an average of 25 U/ml of enzyme over 20 cycles of repeated batch operation and did not show any decline in production
upon reuse. The enzyme yield correlated well with the biomass content in the beads. Continuous production of the enzyme in PBR
was studied at different substrate concentrations and dilution rates. In general, the volumetric productivity increased with increased
dilution rate and substrate concentrations and the substrate conversion efficiency declined. The PBR operated under conditions
giving maximal substrate conversion efficiency gave an average yield of 21.07 U/ml and an average productivity of 13.49 U/ml/h.
The system could be operated for 120 h without any decline in productivity
Jissa, Krishna G; Dr.Chandrasekaran,M(Cochin University of Science and Technology, January , 2008)
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Abstract:
The marine microorganisms are yet to be exploited as a source of natural
pigments for probable utilization in various industries. Hence, in this study focus
was made only on pigment producing marine bacteria for pigment production and
evaluation of the same for some application besides development of an ideal
bioprocess for subsequent indigenous production of the pigment using the same
organism towards ultimate industrial application.
Description:
Department of Biotechnology, Cochin
University of Science and Technology
Dasan,E V; Chandrasekaran, M(Department of Biotechnology, Faculty of Science, January , 2002)
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Abstract:
Phosphate (Pi) is one among the most important essential residues in maintenance and inheritance of life, with far diverse physiological role as structural, functional and energy transduction. Phosphate accumulation in wastewaters containing run off of fertilizers and industrial discharges is a global problem that results in algal blooms in bays, lakes and waterways. Currently available methods for removing phosphates from wastewater are based primarily on polyP accumulation by the activated sludge bacteria. PolyP plays a critical role in several environmental and biotechnological problems. Possible relation of interaction between polyP accumulation phenomenon, the low biomass, low Pi uptake, and varying results obtained in response to the impact of sodium chloride, pH, temperature, various inorganic salts and additional carbon sources studied, are all intriguing observations in the present investigation. The results of the present study have evidenced very clearly the scope for potential strains of bacteria from both sea water and marine sediments which could be exploited both for Pi removal in wastewater released by industries and intensive aquaculture practices in to the aquatic environment as well as to harness the potential strains for industrial production of polyP which was wide range of applications.