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Please use this identifier to cite or link to this item: http://purl.org/purl/930

Title: L-Glutaminase production by an immobilized marine Pseudomonas sp. BTMS -51
Authors: Rajeev Kumar, S
Chandrasekaran, M
Keywords: Pseudomonas sp. BTMS-51
Ca-alginate entrapment
physical adsorption
polyurethane foam (PUF)
Extra Cellular L-glutaminase
Issue Date: Jun-2001
Publisher: Department of Biotechnology
Abstract: The present study is about the Pseudomonas sp. BTMS-51 isolated from the marine sediments of Cochin Coast. In the present study, it is concluded that marine bacteria are ideal candidates for immobilization using either Ca-alginate entrapment or physical adsorption on to synthetic inert supports and the process of immobilization does not negatively influence them. Thus, Ca-alginate entrapment of the bacteria was found to be well suited for reuse of the biomass and extended operational stability during continuous operation. Adherence of the bacterium to inertsupports was observed to be strong and it imparted minimal stress on the immobilized bacterium and allowed detachment and relocation on the supports which enabled the formation of a dynamic equilibrium maintaining a stable cell loading. This is particularly desirable in the industry for extended operational stability and maintenance of consistently higher outputs. Marine Pseudomonas sp. BTMS-51 is ideal for industrial production of extra cellular L-glutaminase and immobilization on to synthetic inert support such as polyurethane foam could be an efficient technique, employing packed bed reactor for continuous production of the enzyme. Temperature and glutamine concentration had significant effects on enzyme production by cells immobilized on polyurethane foam (PUF).
URI: http://dyuthi.cusat.ac.in/purl/930
Appears in Collections:Faculty of Sciences

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