DSpace About DSpace Software
 

Dyuthi @ CUSAT >
e-SCHOLARSHIP >
Bio Technology >
Faculty >
Dr.Padma Nambisan >

Please use this identifier to cite or link to this item: http://purl.org/purl/733

Title: DNA polymorphism in Cab locus of tomato induced by tissue culture
Authors: Padma, Nambisan
Keywords: hybrid heterozygous
anthocyaninless
somaclonal variation
DNA polymorphism
somaclones
Issue Date: Mar-1992
Abstract: Plants were regenerated from callus induced from leaf disc explants of a tomato F, hybrid heterozygous for three marker loci (a), without anthocyanin (aw), and hairless (hl). Regenerants were studied for somaclonal variation at the phenotypic level by scoring for variation in the marker loci, and at the DNA level by probing geomic DNA blots with a chlorophyll a/b binding protein (Cab-3C) cDNA sequence. While no variation was observed at the phenotypic level in over 950 somaclones studied, DNA polymorphism for the Cab locus could be detected in two out of 17 somaclones tested. Tissue culture induced variation at the phenotypic level for specific loci is very low (less than 0.001 for a, awor hl) but DNA sequence changes are induced at much greater frequency (- 0.1 for a multicopy gene family such as Cab).
URI: http://dyuthi.cusat.ac.in/purl/733
Appears in Collections:Dr.Padma Nambisan

Files in This Item:

File Description SizeFormat
Padma nambisan and others,DNA polymorphism..,mar1992.PDFpdf2.56 MBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback