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Please use this identifier to cite or link to this item: http://purl.org/purl/4244

Title: Continuous production of L-glutaminase by an immobilized marine Pseudomonas sp BTMS-51 in a packed bed reactor
Authors: Chandrasekaran, M
Rajeev Kumar, S
Keywords: Glutaminase
Packed bed reactor
Marine bacteria
Continuous fermentation
Issue Date: 2003
Publisher: Elsevier
Abstract: A marine Pseudomonas sp BTMS-51, immobilized by Ca-alginate gel entrapment was used for the production of extracellular Lglutaminase under repeated batch process and continuous process employing a packed bed reactor (PBR). Immobilized cells could produce an average of 25 U/ml of enzyme over 20 cycles of repeated batch operation and did not show any decline in production upon reuse. The enzyme yield correlated well with the biomass content in the beads. Continuous production of the enzyme in PBR was studied at different substrate concentrations and dilution rates. In general, the volumetric productivity increased with increased dilution rate and substrate concentrations and the substrate conversion efficiency declined. The PBR operated under conditions giving maximal substrate conversion efficiency gave an average yield of 21.07 U/ml and an average productivity of 13.49 U/ml/h. The system could be operated for 120 h without any decline in productivity
Description: Process Biochemistry 38 (2003) 1431 /1436
URI: http://dyuthi.cusat.ac.in/purl/4244
Appears in Collections:Dr. M Chandrasekharan

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